Multi-functional 8-channel Electronic Pipette (dPette+)

Multi-functional 8-channel Electronic Pipette (dPette+) 

PRIMARY CLINICAL & DIAGNOSTIC USES

1. High-Throughput Sample Preparation for PCR and qPCR Assays

• Primary Use: Provides a motor-driven, 8-channel electronic pipette designed for rapid, precise, and simultaneous liquid handling across all eight channels, significantly accelerating sample preparation for high-throughput molecular diagnostics such as PCR and qPCR workflows.

• How it helps: For the molecular biology technologist, the 8-channel design allows for the simultaneous loading of 96-well plates, drastically reducing the time required for PCR reaction set-up. According to comparative studies, the dPette+ can fill a 96-well plate in under 3 minutes, which is a 60% increase in efficiency compared to manual multichannel pipettes. Its high-precision stepper motor ensures consistent volume delivery across all channels, which is critical for the reproducibility of diagnostic tests. For the patient, this translates directly to more reliable PCR results, reducing the likelihood of false negatives or inconclusive results, and enabling faster treatment decisions.

2. Consistent Reagent Dispensing for ELISA and Immunoassays

• Primary Use: Engineered for the precise and consistent dispensing of reagents, wash buffers, and substrates across 96-well microplates, which is fundamental to the accuracy of enzyme-linked immunosorbent assays (ELISA) and other immunoassays.

• How it helps: For the clinical laboratory scientist, the multiple functional modes—specifically the “continuous dispensing” and “sequential dispensing” modes—allow for the automated and error-free addition of samples and reagents, reducing operator fatigue and variability. The adjustable speed control prevents splashing and foaming, preserving the integrity of delicate immunological reactions. For the patient, this automation ensures that every well on the plate receives exactly the same treatment, leading to highly reproducible results, which is critical for accurate disease diagnosis (e.g., HIV, Hepatitis) and therapeutic drug monitoring.

3. Multi-Sample Liquid Transfer for Cell Culture and Drug Discovery

• Primary Use: Streamlines the transfer of cell suspensions, culture media, and drug compounds in cell-based assays, toxicology screening, and pharmaceutical research laboratories.

• How it helps: For the research scientist and pharmaceutical technician, the 8-channel capability is ideal for seeding cells into 96-well plates for high-throughput screening. The “Mixing” mode can be used to resuspend cell pellets or homogenize drug compounds before dispensing, eliminating an extra manual step. The lightweight, ergonomic design minimizes the risk of repetitive strain injuries (RSI) during long hours of repetitive pipetting. For the patient, this efficiency and safety accelerate the R&D of new therapies and vaccines, ensuring that potentially life-saving treatments reach the clinic faster.

4. Simultaneous Micro-Volume Transfer for Next-Generation Sequencing (NGS)

• Primary Use: Capable of accurately transferring micro-volumes of complex NGS library preparation reagents (e.g., adapters, indexes, and master mixes) across 96 and 384-well plates.

• How it helps: For the genomics specialist, the 8-channel dPette+ offers a range of models from 0.5-10 μL to 30-300 μL, providing the precision needed for microliter-scale NGS workflows. The stepper motor ensures the synchronous movement of all eight pistons, guaranteeing uniform liquid transfer across each column of the plate. The device also supports user self-calibration via USB, ensuring that the instrument maintains its high accuracy over time without costly third-party delays. For the patient, this precision in NGS library preparation results in more uniform sequencing coverage and higher-quality genetic data, leading to more accurate diagnosis of hereditary diseases and cancer biomarkers.

5. Versatile High-Throughput Clinical Diagnostics

• Primary Use: Allows clinical laboratories to rapidly process and transfer patient samples (e.g., serum, plasma, urine) into multi-well plates for a variety of automated analyzers and diagnostic platforms.

• How it helps: For the clinical laboratory manager, the pipette’s dual charging (USB and stand) capability ensures that the device is always ready for continuous operation, minimizing downtime during critical testing rushes. The ability to autoclave the lower section also allows for easy sterilization, preventing cross-contamination between batches of patient samples. For the patient, this results in a more efficient laboratory workflow, dramatically reducing the turnaround time for critical test results and supporting high-volume screening programs for infectious diseases.

SECONDARY & SUPPORTIVE USES

1. Solution Preparation and Serial Dilutions: Used to create standard curves and perform precise serial dilutions for quantitative assays, ensuring accurate concentration gradients for drug testing.

2. 96-Well and 384-Well Plate Filling: The primary tool for filling multi-well plates in high-throughput screening (HTS) labs, used in drug discovery and genomics research.

3. Master Mix Distribution for Genomics: Ideal for distributing PCR master mix into 96-well plates before the addition of individual DNA samples in genotyping and sequencing workflows.

4. Bacterial and Yeast Culture Inoculation: Used for the rapid inoculation of multiple culture wells or tubes with microbial cultures for antimicrobial susceptibility testing (AST).

5. Microplate Coating for Immunoassays: Employed in the application of capture antibodies and blocking buffers during the manufacturing of ELISA kits.

6. Biobanking and Sample Aliquoting: Facilitates the rapid splitting of large-volume biological samples (e.g., plasma, urine) into multiple replicate tubes or plates for long-term storage.

7. Cell Viability Assays (MTT/XTT): Accurately dispenses MTT or other tetrazolium dyes into multi-well plates for cell proliferation and cytotoxicity testing.

8. Protein Crystallization Screening: Used to dispense precise volumes of protein solutions, precipitants, and additives into crystallization plates for structural biology research.

9. Quality Control (QC) Transfer in Pharma: Transfers raw materials, intermediates, and finished product samples from flasks or vials into HPLC autosampler vials for QC testing.

10. Teaching and Training: Used in educational laboratories to train students in high-throughput liquid handling techniques.

11. Flow Cytometry Sample Preparation: Used to dispense antibodies for cell staining protocols in flow cytometry.