Enzymatic Reaction

The Pro Thermo Mix (HCM100) is a programmable digital thermomixer (thermal mixer) that integrates heating, cooling, and orbital mixing functions in a single compact benchtop instrument, designed for comprehensive sample preparation in molecular diagnostics, clinical chemistry, and research laboratories . With a temperature range from 0°C to 100°C (heating to 100°C, cooling to 15°C below room temperature), temperature control accuracy of ±0.5°C, and orbital mixing speed range of 200-1500 rpm (3mm orbit), this thermomixer provides precise, uniform temperature control and consistent sample agitation for a wide range of diagnostic and research applications .

The HCM100-Pro features a microprocessor-controlled system with full-color TFT display for real-time monitoring of temperature, time, and mixing speed, programmable memory supporting up to 9 custom programs, rapid heating (5.5°C/min) and controlled cooling (5°C/min from 100°C to ambient, 0.5°C/min below ambient) capabilities, and automatic block recognition . The magnetic adsorption technology allows tool-free, secure mounting of interchangeable aluminum heating blocks compatible with various tube formats including 0.2mL PCR tubes, 0.5mL, 1.5mL, 2mL, 5mL, 15mL, 50mL centrifuge tubes, and microplates . The included block lid provides heat preservation, temperature stability, and contamination prevention .

For the molecular biologist and clinical laboratory scientist, the HCM100-Pro supports nucleic acid extraction and purification (DNA/RNA) protocols requiring controlled temperature incubation and mixing, enzymatic reactions (restriction enzyme digestions, ligations, PCR preparation), protein denaturation for Western blotting and SDS-PAGE, solid phase extraction (SPE) applications, gene synthesis and purification, and bacterial culture preparation . The active cooling capability (to 15°C below ambient) enables rapid cooling of samples after high-temperature processing, preserving heat-sensitive DNA, RNA, enzymes, and proteins for downstream molecular diagnostic workflows .

Safety features include over-temperature protection (150°C safety cut-off) , built-in temperature calibration function for long-term accuracy , automatic block recognition , and cTUVus and CE safety certifications . For the patient undergoing diagnostic testing for infectious diseases, genetic conditions, or oncology biomarkers, the HCM100-Pro's precise temperature control (±0.5°C accuracy) and integrated heating/cooling/mixing capabilities ensure reliable nucleic acid extraction, consistent enzymatic reaction conditions, and accurate sample preparation—supporting reliable test results that guide appropriate treatment decisions across clinical chemistry, molecular diagnostics, and research applications .