Pro Multifunctional Orbital Decolorizing Shaker

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The SK-O330-Pro Large Multifunctional Orbital Decolorizing Shaker (also known as the LCD Digital Orbital Laboratory Shaker) is a large-capacity benchtop orbital shaker designed for high-throughput gel staining and destaining, Western blot washing, hybridization, RPR/TRUST syphilis testing, and general sample mixing in clinical diagnostic and research laboratories . Operating with a smooth orbital motion at 10mm amplitude and adjustable speeds from 100-500 rpm, this digital shaker supports a maximum load capacity of 7.5 kg with a maintenance-free brushless DC motor that requires no routine servicing .

For the clinical laboratory scientist and medical technologist, the SK-O330-Pro facilitates high-volume standardized RPR card testing for syphilis diagnosis using an optional transparent top cover and thermal insulation device . Its larger 320×320mm work surface accommodates multiple staining trays, allowing batch processing of protein gels for clinical electrophoresis workflows. The shaker supports DNA/RNA hybridization for molecular diagnostics, Western blot membrane washing for protein detection, and gentle mixing for cell culture applications at temperatures as low as 4°C . It is compatible with a wide range of platforms and clamps for culture flasks, Erlenmeyer flasks, beakers, staining trays, and other laboratory vessels .

The shaker features dual independent backlit LCD displays for real-time monitoring of speed and time, with programmable run times from 1 minute to 99 hours 59 minutes or continuous operation . The RS232 data interface enables PC connectivity using free ShakerPC software, allowing users to set instrument parameters, log data, and display real-time work curves from a computer . The wide voltage tolerance (100-240V) ensures compatibility with laboratory power supplies worldwide.

Safety features include over-speed detection and protection, automatic speed regulation, IP21 protection rating, and a maintenance-free brushless motor design that eliminates brush replacement . The SK-O330-Pro holds cTUVus and CE safety certifications and is compatible with cold rooms (4°C), incubators, and standard laboratory environments .

With a compact footprint (approximately 370×420×100 mm) despite its high capacity, and weighing 12 kg, the SK-O330-Pro provides versatile, high-throughput orbital shaking performance for routine laboratory workflows supporting patient diagnosis and biomedical research

Description

Pro Multifunctional Orbital Decolorizing Shaker

PRIMARY CLINICAL & DIAGNOSTIC USES

1. Syphilis Serology Testing (RPR/TRUST)

  • Primary Use: Provides consistent large-platform orbital shaking for rapid plasma reagin (RPR) and toluidine red unheated serum test (TRUST) syphilis screening, with a working tray that can be equipped with an optional transparent top cover and built-in thermal insulation device for placing sponges specifically for these tests .

  • How it helps: For the medical technologist and clinical laboratory scientist, the SK-O330-Pro ensures standardized agitation for RPR card tests with a larger work area, allowing batch processing of multiple patient samples simultaneously, reducing inter-run variability, and improving reproducibility of qualitative and semi-quantitative syphilis results. For the patient, standardized serological testing contributes to accurate diagnosis of syphilis infection, enabling appropriate antibiotic treatment and preventing disease progression.

2. Gel Staining and Destaining for Protein Analysis

  • Primary Use: Accommodates large staining trays and containers for Coomassie Blue and silver staining of protein gels following electrophoresis, providing smooth orbital motion (10mm amplitude) for uniform reagent coverage and efficient destaining .

  • How it helps: For the clinical biochemist and laboratory scientist, the SK-O330-Pro’s 10mm orbital amplitude and 100-500 rpm speed range enable even staining and rapid destaining of multiple polyacrylamide gels simultaneously—improving band visualization and reducing background interference. The larger 320×320mm work plate accommodates gels of various sizes for high-throughput protein analysis. For the patient, reliable protein analysis supports diagnosis of hemoglobinopathies, multiple myeloma, and other protein disorders where accurate gel interpretation guides clinical management.

3. DNA Hybridization and Molecular Assays

  • Primary Use: Used for Southern blot, Northern blot, and Western blot hybridization steps, ensuring uniform probe distribution and consistent washing conditions for membrane-based assays .

  • How it helps: For the molecular biologist and clinical laboratory scientist, the SK-O330-Pro’s digital speed control, timer function (1 min-99 hr 59 min), and larger capacity enable reproducible hybridization and stringent wash steps for multiple blots simultaneously—improving signal-to-noise ratios and reducing non-specific binding . For the patient undergoing molecular genetic testing, reliable hybridization results contribute to accurate detection of genetic mutations, infectious agents, or cancer biomarkers.

4. General Large-Volume Sample Mixing and Reagent Preparation

  • Primary Use: Facilitates mixing of multiple blood collection tubes, large-volume reagent preparations, buffer solutions, and liquid cultures prior to analysis or further processing, with a maximum load capacity of 7.5 kg .

  • How it helps: For the clinical laboratory scientist, the shaker’s continuous or timed operation modes and larger work surface (320×320mm) ensure consistent mixing of anticoagulated blood samples for hematology testing and homogeneous reagent suspensions for automated analyzers—improving pre-analytical quality while processing more samples per run. For the patient, proper sample mixing before analysis reduces result variability and the likelihood of repeat specimen collection.

SECONDARY & SUPPORTIVE USES

1. Cell Culture and Bacterial Growth: Used for gentle shaking of multiple cell culture flasks and bacterial cultures simultaneously in incubators and cold rooms (operating temperature range: 4-40°C) .

2. Immunoassays: Supports ELISA plate washing and incubation steps for high-throughput workflows, providing consistent agitation for antigen-antibody binding reactions.

3. Extraction Procedures: Used for solvent extraction, liquid-liquid extraction, and solid-phase extraction workflows in clinical chemistry and toxicology laboratories.

4. Western Blot Membrane Washing: Supports washing and blocking steps for multiple Western blotting membranes in protein detection workflows.

5. Histology and Cytology Staining: Supports staining and destaining protocols for large batches of histological slides and cytological specimens.

6. Pharmaceutical Quality Control: Used in QC laboratories for dissolution testing, sample homogenization, and large-volume reagent mixing.

7. Environmental Testing: Supports water and soil sample extraction and mixing for environmental microbiology and chemistry applications.

8. Research and Academic Laboratories: Used in university and research laboratory settings for molecular biology, biochemistry, and microbiology high-throughput workflows .

9. Food and Beverage Testing: Supports sample preparation for microbiological and chemical analysis in food safety testing.

KEY PRODUCT FEATURES

1. BASIC IDENTIFICATION ATTRIBUTES

  • Device Type: A large-capacity benchtop orbital shaker with dual LCD digital control, maintenance-free brushless DC motor, and RS232 PC connectivity, designed for decolorizing, staining, hybridization, and general mixing in clinical diagnostic and research laboratories requiring higher throughput .

  • Designation: SK-O330-Pro Large Multifunctional Orbital Decolorizing Shaker, SK-O330-Pro Orbital Shaker, LCD Digital Orbital Laboratory Shaker, Large Orbital Shaker, DLAB SK-O330-Pro, SCILOGEX SK-O330-Pro .

  • Motion Type: Orbital/Circular shaking motion with 10mm amplitude .

  • Key Components:

    • Large work platform (320 × 320 mm) with wide range of optional clamps and platforms for flasks, beakers, and other vessels

    • Dual independent LCD display panels for speed and time

    • RS232 data interface for PC connection

    • Optional transparent top cover for RPR/TRUST testing with built-in thermal insulation device

  • Motor: Maintenance-free brushless DC motor, 28W input, 15W output .

  • Drive System: Microprocessor-controlled with digital feedback for precision speed regulation.

2. TECHNICAL & PERFORMANCE PROPERTIES

  • Maximum Load Capacity: 7.5 kg (including clamps and fixtures) .

  • Speed Range: 100 – 500 rpm .

  • Orbit Diameter: 10 mm .

  • Speed Display: Dual independent backlit LCD display for speed and timer .

  • Timer Range: 1 minute to 99 hours 59 minutes (some sources indicate up to 19 hours 59 minutes); continuous "HOLD" operation also available .

  • Timer Display: Independent LCD .

  • Operating Modes: Continuous run mode; timed operation mode .

  • Motor Type: Maintenance-free brushless DC motor .

  • Motor Input/Output Power: 28W (input), 15W (output) .

  • Instrument Power: 30W .

  • Power Requirements: AC 100-240V, 50/60Hz (wide voltage compatibility) .

  • Data Interface: RS232 for PC control and data logging .

  • Software: Free ShakerPC software available for download, enabling instrument parameter setting and real-time work curve display on computer .

  • Operating Temperature: 5 – 40°C; cold room compatible down to +4°C .

  • Permissible Humidity: 80% RH .

  • Dimensions (W × D × H): 370-420 × 370-420 × 100 mm (manufacturer: 370×420×100mm) .

  • Work Platform Dimensions (W × D): 320 × 320 mm .

  • Weight: 12-13.5 kg (manufacturer: 12 kg) .

  • Protection Class: IP21 .

  • Safety Certifications: cTUVus approved, CE Marked .

3. PHYSICAL & OPERATIONAL PROPERTIES

  • Construction: Large benchtop design with stable, low-profile housing for enhanced stability during high-load operation.

  • Body Material: High-impact durable housing with corrosion-resistant finish.

  • Control Panel: Dual independent backlit LCD displays with user-friendly membrane keypad and separate knobs for speed and time adjustment .

  • Display Features: Separate LCD screens for speed (rpm) and time; real-time parameter display; large, easy-to-read format.

  • Operation Modes: Continuous run; timed run with audible completion alert; PC-controlled operation via RS232 .

  • Shaking Action: Smooth, uniform 10mm orbital motion providing thorough mixing and even reagent coverage across large work surface .

  • Safety Features: Over-speed detection and protection; automatic speed regulation; maintenance-free brushless motor .

  • Remote Function: Full PC control and data transmission via RS232 interface with free ShakerPC software download .

  • Application Flexibility: Wide range of optional platforms available for culture flasks, Erlenmeyer flasks, beakers, staining trays, clinical test tubes, and other vessels .

4. SAFETY & COMPLIANCE ATTRIBUTES

  • Regulatory Status: General laboratory equipment meeting international safety standards.

  • Safety Certifications: cTUVus certified; CE Marked; compliant with applicable EMC and safety directives .

  • Electrical Safety: Wide voltage tolerance (100-240V); overcurrent protection; over-speed detection; IP21 protection rating against solid objects >12mm and vertically falling water drops .

  • Safety Features: Over-speed detection and protection mechanism; stable platform locking; non-skid rubber feet for vibration dampening.

  • Environmental Compatibility: Designed for use in cold rooms (4°C minimum), incubators, and standard laboratory environments .

5. STORAGE & HANDLING ATTRIBUTES

  • Storage: Store on clean, dry, level benchtop with adequate clearance for ventilation and vibration isolation.

  • Cleaning: Wipe exterior with soft, damp cloth; use mild detergent if needed; avoid liquid ingress into housing (IP21 protection limits exposure).

  • Platform Maintenance: Remove and clean platforms with mild detergent; dry thoroughly before reinstallation; inspect for wear or damage.

  • Inspection: Inspect platform, attachments, and fastening mechanisms for damage before each use; ensure all clamps are secure.

  • Power Cord: Inspect power cord for damage before each use; ensure proper grounding.

  • Calibration: Factory calibrated; user calibration available via PC software if needed.

  • Software Updates: Free ShakerPC software available for download from manufacturer website.

6. LABORATORY & CLINICAL APPLICATIONS

  • Primary Application: High-throughput gel staining and destaining, Western blot membrane washing, decolorizing, hybridization, RPR/TRUST syphilis testing, and general sample mixing for molecular biology, biochemistry, and clinical diagnostic workflows requiring larger capacity.

  • Clinical Role: Essential laboratory equipment in clinical diagnostic laboratories, serology laboratories performing high-volume syphilis screening, molecular diagnostics labs, clinical biochemistry labs, histology labs, research laboratories, and pharmaceutical QC facilities

SAFETY HANDLING PRECAUTIONS

1. SAFETY PRECAUTIONS

General Safety Instructions

Read the operating instructions completely before use and follow the safety instructions. Ensure that only trained staff work with the instrument .

Protective Grounding

Make sure the socket must be earthed (protective ground contact) before use .

Personal Protective Equipment (PPE)

Wear the personal protective equipment in accordance with the hazard category of the media to be processed. Hazards include splashing of liquids, vibration force leading to broken glass containers, and entanglement of body, hair, clothes, or jewelry .

Explosive Atmospheres & Hazardous Materials

Do not operate the instrument in explosive atmospheres, with hazardous substances, or under water. Working with combustible media or flammable materials may result in additional hazards. Only process media that will not react dangerously to the extra energy produced through processing .

Installation & Work Surface

Set up the instrument in a spacious area on a stable, clean, non-slip, dry, and fireproof surface. Do not cover the instrument. Protect the instrument and accessories from bumps and impacts .

  • Environmental Conditions: Permissible ambient temperature: 5-40°C; cold room compatible down to 4°C. Allowable relative humidity: 80% RH .

  • Load Capacity: Maximum load capacity (including clamps): 7.5 kg. Do not exceed this limit .

  • Secure Accessories & Vessels: Accessories must be securely attached to the instrument so that they cannot come off by themselves, and vessels must be firmly placed on the shaking table to avoid any injury .

  • Speed Selection: Observe the vessel on the shaking table for medium splashing out of liquid when setting the motor speed. Reduce the motor speed if the instrument is not running smoothly. Start with lower speeds when using heavy loads or large vessels; gradually increase to desired speed. Adjust the motor speed knob slowly in order to keep the instrument running smoothly .

  • Moving Parts: Users are not allowed to touch moving parts of the instrument to avoid any accidents. Pay attention to protect hands or fingers from rolling when moving the instrument .

  • Operation Monitoring: The shaker has safe speed protection (over-speed detection). If the instrument is not running smoothly, reduce motor speed immediately .

  • Maintenance Access: The instrument may only be opened by trained technicians. Always disconnect the plug beforehand .

  • Accessory Installation: Always disconnect the plug before fitting accessories .

  • Pre-Use Inspection: Check the instrument and accessories beforehand for damage each time you use them. Do not use damaged components .

  • Magnetic Fields: Keep away from high magnetic fields .

2. FIRST AID MEASURES

  • Sample Spill: If sample spills onto instrument, stop operation immediately, unplug unit, clean and disinfect affected area thoroughly with appropriate disinfectant.

  • Instrument Malfunction: If irregular noise or vibration occurs, stop instrument immediately, check load balance and platform security.

  • Power Failure: If power fails during operation, turn off mains switch; after power is restored, reset any timed parameters as they may be erased .

  • Over-speed Alarm: If over-speed detection activates, reduce set speed and restart; ensure load is within specifications and evenly distributed.

  • Tube/Bottle Breakage: If glass vessel breaks during operation, stop shaker immediately, carefully remove fragments using forceps while wearing cut-resistant gloves, disinfect platform and surrounding area.

3. FIRE FIGHTING MEASURES

  • Flammability: Plastic housing and electrical components present fire risk.

  • Extinguishing Media: Use carbon dioxide or dry chemical extinguisher for electrical fires.

  • Fire Response: Unplug instrument immediately if safe to do so; do not use water on electrical fire.

  • Prevention: Keep flammable materials away from instrument during operation; ensure adequate clearance for ventilation.