Transfer electrophoresis tank (DL-ZY03)

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The Transfer electrophoresis tank (DL-ZY03) is a large-format, high-capacity electroblotting (wet transfer) system designed for high-throughput Western, Southern, and Northern blotting in clinical diagnostic and research laboratories . Capable of transferring up to four mini-gels (or two large gels) simultaneously, this system dramatically increases the throughput of confirmatory diagnostic testing for infectious diseases (HIV, Hepatitis, Lyme) and autoimmune disorders .

Manufactured from highly transparent, impact-resistant polycarbonate and featuring 99.99% pure platinum electrodes, the Transfer electrophoresis tank (DL-ZY03) generates a powerful, uniform electric field for efficient, consistent transfer of proteins and nucleic acids of all sizes . A large built-in ice box and two blue cooling units provide superior thermal management, preventing protein denaturation and ensuring sharp, well-resolved bands critical for diagnostic interpretation .

The dual independent transfer cassettes, user-friendly open-ended gel trays, and included bubble roller streamline the assembly process and reduce technical variability . The system’s robust construction, safety features to prevent incorrect polarity connection, and compatibility with standard mini and large-format gels make it an essential tool for reference laboratories, hospital central labs, and biopharmaceutical QC facilities performing high-volume protein characterization and confirmatory diagnostics .

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Category: LABORATORY EQUIPMENT AND SUPPLIES Tags: Clinical Laboratory Equipment, DL-ZY03 Transfer Electrophoresis Tank, DLAB Transfer Tank, High-Throughput Western Blot, Immunoblotting, Large Format Electroblotting, Membrane Transfer, Protein Transfer Tank, Western Blot Transfer

Description

Transfer electrophoresis tank (DL-ZY03)

PRIMARY CLINICAL & DIAGNOSTIC USES

1. High-Throughput Western Blot Protein Transfer for Confirmatory Diagnostic Testing

Primary Use: Provides a large-format electroblotting (transfer) tank designed for the rapid and simultaneous transfer of up to four mini-gels (or two large gels) of proteins onto PVDF or nitrocellulose membranes for Western blot-based confirmatory diagnostic testing .
How it helps: For the clinical immunologist and molecular diagnostics laboratory manager, the Transfer electrophoresis tank (DL-ZY03) expanded capacity enables high-throughput processing of Western blot confirmatory tests for infectious diseases (HIV, Hepatitis, Lyme disease) and autoimmune disorders (Lupus, Myositis). For the patient, higher throughput reduces turnaround times for these crucial confirmatory tests, enabling faster clinical decision-making and treatment initiation.

2. Nucleic Acid Transfer for Southern and Northern Blotting in Genetic Diagnostics

Primary Use: Facilitates the efficient transfer of DNA (Southern Blot) and RNA (Northern Blot) fragments from large agarose gels to membranes for hybridization-based detection of specific genetic sequences and gene expression analysis .
How it helps: For the molecular geneticist, the Transfer electrophoresis tank (DL-ZY03) large membrane capacity supports complex genetic diagnostics, including detection of large gene rearrangements, triplet repeat expansions (Huntington’s disease), and genomic DNA analysis for cancer biomarker discovery. For the patient, this capability is essential for diagnosing inherited disorders where large gene fragments must be analyzed and for research that leads to new targeted therapies.

3. Powerful and Uniform Protein Transfer with Optimized Electrode Design

Primary Use: Features a high-performance electrode system that generates a uniform, high-strength electric field across the entire transfer area, ensuring rapid and even transfer of proteins of all molecular weights, from small peptides to large complexes .
How it helps: For the laboratory scientist, the uniform field ensures that proteins from across the entire gel are transferred consistently, eliminating incomplete transfer or “smiling” effects which can compromise diagnostic band interpretation. For the patient, a stable, efficient transfer guarantees that faint but clinically significant antibody bands are not missed, ensuring the highest diagnostic sensitivity for conditions like Lyme disease or HIV seroconversion.

4. Simultaneous Dual-Tray Processing for Maximum Laboratory Efficiency

Primary Use: Capable of transferring up to four mini-gels (83 × 73 mm) or two large-format gels simultaneously, dramatically increasing the throughput of Western blot workflows .
How it helps: For the clinical laboratory manager, this capacity allows for the processing of a full day’s worth of patient samples in a single run, drastically reducing energy costs, buffer usage, and technician hands-on time. For the patient, consolidated processing results in a shorter time from sample receipt to final diagnostic report, expediting clinical care pathways.

5. Superior Thermal Management for High-Resolution Diagnostic Blots

Primary Use: Integrates a large internal ice box and blue cooling units to rapidly dissipate the heat generated during high-intensity transfers, preventing protein denaturation and ensuring sharp, well-resolved bands .
How it helps: For the laboratory scientist, the robust thermal management prevents the formation of “smiling” bands and membrane distortion, which is crucial for accurate band interpretation, especially when distinguishing multiple bands of similar molecular weight (e.g., in HIV or Lyme disease Western blots). For the patient, maintaining a stable, cool transfer environment ensures the diagnostic blot produces a clear, interpretable pattern, reducing the risk of equivocal or false-positive results.

SECONDARY & SUPPORTIVE USES

1. Automated and High-Throughput Immunoblotting: Can be integrated into automated workflows for large-scale immunoblot screening for autoimmune diseases (e.g. Systemic Sclerosis) .

2. Quality Control of Complex Biologics: Used in Western blot analysis for purity, identity, and stability testing of monoclonal antibodies, fusion proteins, and viral vectors used in gene therapy .

3. Characterization of Protein Aggregates: Supports the transfer of proteins separated under non-denaturing conditions for detection of soluble and insoluble protein aggregates in neurodegenerative disease research (e.g., Tau, alpha-synuclein) .

4. Phosphoproteomics and Post-Translational Modification Analysis: Used for transferring proteins for probing with specific antibodies against phosphorylation, acetylation, and ubiquitination sites in cancer signaling research .

5. Process Analytical Technology (PAT) in Biomanufacturing: Used in pilot plants for in-process testing of harvest and purification steps to ensure product quality .

6. Cardiovascular Disease Biomarker Discovery: Supports large-scale validation studies of candidate protein biomarkers for coronary artery disease and heart failure .

7. Plant Pathology and Agricultural Research: Used for the detection of plant viral proteins and host-response proteins in GM crop research .

8. Neurodegenerative Disease Confirmation: Used as a confirmatory test for detecting pathological protein conformers (e.g., abnormal prion protein, phosphorylated tau) in cerebrospinal fluid (CSF) .

9. Detection of Rare Protein Variants in Genetic Screening: Supports the transfer of proteins for immunodetection in research on monogenic disorders affecting protein expression .

10. Veterinary Diagnostic Confirmatory Testing: Used in reference labs for confirmatory Western blot testing for diseases like Equine Infectious Anemia (EIA) and Feline Immunodeficiency Virus (FIV) .

11. Environmental and Food Allergen Testing: Used for the high-sensitivity detection of trace food allergens (e.g., peanut, gluten) in processed food products .

12. Clinical Assay Validation: Used in the validation and verification of new commercial Western blot kits for a chosen clinical marker .

KEY PRODUCT FEATURES

1. BASIC IDENTIFICATION ATTRIBUTES

Device Type: A high-capacity, large-format wet transfer system for the electroblotting (electrotransfer) of proteins and nucleic acids from gels to membranes for Western, Southern, and Northern blotting in high-throughput clinical and research applications .
Designation: DL-ZY03 Transfer Electrophoresis Tank, High-Throughput Electroblotting System, Large-Format Western Blot Transfer Cell, DLAB DL-ZY03, High-Capacity Membrane Transfer Tank .
Key Components:
- Tank Body: Large-format, highly transparent polycarbonate tank for real-time monitoring .
- Electrode Core: 99.99% pure platinum wire electrodes for optimal conductivity and durability .
- Dual Transfer Cassettes: Two independent cassettes, each capable of holding up to two mini-gels, enabling simultaneous processing of four blots .
- Gel Holder Trays: User-friendly, open-ended trays for easy assembly of the gel-membrane sandwich .
- Sponge Pads: High-quality foam pads (included) to ensure uniform pressure on the gel-membrane "sandwich" for even transfer .
- Cooling System: An integrated, large-capacity ice box and two blue cooling units (included) for superior thermal management .
- Bubble Roller: A special roller tool included to remove air bubbles from the gel-membrane sandwich before transfer .

2. TECHNICAL & PERFORMANCE PROPERTIES

Transfer Area per Cassette: 110 × 90 mm .
Gel Compatibility: Compatible with both mini-gels (83 × 73 mm) and large-format gels .
Maximum Capacity: Capable of transferring up to 4 mini-gels (83 × 73 mm) or 2 large gels per run .
Electrode Material: 99.99% high-purity platinum (Pt) wire, φ0.25mm, for excellent conductivity and corrosion resistance .
Electrical Design: Generates a uniform, high-strength electric field across the tank for efficient, consistent transfer .
Construction Material: Highly transparent, durable, and chemically resistant polycarbonate (PC) .
Safety: Incorporates design features to prevent incorrect polarity connection .
Dimensions (W×D×H): 270 × 120 × 160 mm (approx.) .
Weight: Approximately 1.7 kg .

3. PHYSICAL & OPERATIONAL PROPERTIES

Construction: Robust, leak-proof design manufactured via high-precision injection molding .
Electrode Quality: 99.99% high-purity platinum electrodes offer superior conductivity, corrosion resistance, and anti-aging properties .
Thermal Management: Features a large built-in ice reservoir and includes two blue cooling units, effectively absorbing heat generated during high-current runs .
Safety Design: Designed with a "limit function" on the upper cover to prevent the polarity from being reversed when connecting to the power supply .
High-Throughput Design: Dual independent transfer cassettes allow for streamlined workflow; one cassette can be loaded while another is transferring .
Compatibility: Designed to work seamlessly with standard mini-gel and large-format electrophoresis systems .
User Experience: Open-ended gel tray design and included bubble roller simplify the assembly of the gel-membrane sandwich, ensuring high-quality, reproducible transfers .

4. SAFETY & COMPLIANCE ATTRIBUTES

Regulatory Status: Medical laboratory equipment for in vitro diagnostic (IVD) use .
Safe Operation: Safety interconnects and cover design prevent operation if not correctly assembled.
Electrical Safety: Designed for safe low-voltage operation when used with a standard electrophoresis power supply .
Material Safety: High-strength, non-toxic polycarbonate material for the tank body .

5. STORAGE & HANDLING ATTRIBUTES

Storage: Store on a clean, dry benchtop. Ensure the tank is clean and dry before storage .
Cleaning: Rinse thoroughly with distilled water after each use. The polycarbonate tank is resistant to common buffers, but avoid harsh organic solvents .
Cooling Unit Care: Freeze the built-in ice box and two blue cooling units thoroughly before each use. Clean and dry after use to prevent mold growth .
Sponge Pad Care: Inspect and rinse sponge pads thoroughly with distilled water after each use to remove salt deposits. Squeeze dry and replace if they become compressed or torn .
Platinum Electrode Care: Handle with extreme care to prevent bending or physical damage. Clean by gentle rinsing with distilled water; never scratch the metal surface .

6. LABORATORY & CLINICAL APPLICATIONS

Primary Application: High-throughput wet electroblotting (electrotransfer) of proteins and nucleic acids for Western blotting, Southern blotting, Northern blotting, and other immunodetection methods in clinical diagnostic, pharmaceutical, and research laboratories .
Clinical Diagnosis: Increased throughput for confirmatory diagnostic testing for infectious diseases (HIV, Hepatitis, Lyme disease), autoimmune disorders, and genetic conditions via immunoblotting and nucleic acid hybridization .

SAFETY HANDLING PRECAUTIONS

1. SAFETY PRECAUTIONS

Correct Polarity: Ensure the transfer cassettes are correctly oriented with the tank's electrode core. The cover features a safety design to prevent incorrect polarity, but always double-check .
Buffer Level: Fill the tank completely with the recommended volume of chilled transfer buffer to fully submerge the transfer cassettes .
Temperature Control: Mandatory use of the pre-frozen ice box and cooling units is critical for high-intensity runs to prevent protein degradation and membrane damage .
Air Bubble Removal: Always use the supplied bubble roller to gently remove all air bubbles between the gel and the membrane. Trapped air bubbles create transfer voids, leading to inaccurate band patterns .
Membrane Handling: Use clean gloves and flat-tipped forceps when handling membranes to prevent contamination .
Electrical Safety: Do not open the lid or touch the buffer while the system is active to avoid electric shock.
Training: Only trained laboratory personnel should operate the DL-ZY03 for clinical diagnostic applications.

2. FIRST AID MEASURES

Buffer Spill: If Transfer Buffer (containing methanol and Tris-glycine) spills on skin, wash off immediately with soap and water . For eye exposure, flush with water for 15 minutes and seek medical attention.
Electrical Shock: Disconnect the power supply immediately and seek medical attention if any shock is received or suspected .
Mechanical Injury: The polycarbonate tank is impact-resistant; however, if breakage occurs, carefully collect broken fragments and dispose of them in a sharps container.

3. FIRE FIGHTING MEASURES

Flammability: Transfer buffer contains methanol, a Class IB flammable liquid. Polycarbonate tank components are combustible .
Extinguishing Media: Use carbon dioxide (CO₂), dry chemical, or alcohol-resistant foam for fires involving the buffer .

Transfer electrophoresis tank (DL-ZY03)

Transfer electrophoresis tank (DL-ZY03)

PRIMARY CLINICAL & DIAGNOSTIC USES

1. High-Throughput Western Blot Protein Transfer for Confirmatory Diagnostic Testing

Primary Use: Provides a large-format electroblotting (transfer) tank designed for the rapid and simultaneous transfer of up to four mini-gels (or two large gels) of proteins onto PVDF or nitrocellulose membranes for Western blot-based confirmatory diagnostic testing .

2. Nucleic Acid Transfer for Southern and Northern Blotting in Genetic Diagnostics

Primary Use: Facilitates the efficient transfer of DNA (Southern Blot) and RNA (Northern Blot) fragments from large agarose gels to membranes for hybridization-based detection of specific genetic sequences and gene expression analysis .

3. Powerful and Uniform Protein Transfer with Optimized Electrode Design

Primary Use: Features a high-performance electrode system that generates a uniform, high-strength electric field across the entire transfer area, ensuring rapid and even transfer of proteins of all molecular weights, from small peptides to large complexes .

4. Simultaneous Dual-Tray Processing for Maximum Laboratory Efficiency

Primary Use: Capable of transferring up to four mini-gels (83 × 73 mm) or two large-format gels simultaneously, dramatically increasing the throughput of Western blot workflows .

5. Superior Thermal Management for High-Resolution Diagnostic Blots

Primary Use: Integrates a large internal ice box and blue cooling units to rapidly dissipate the heat generated during high-intensity transfers, preventing protein denaturation and ensuring sharp, well-resolved bands .

SECONDARY & SUPPORTIVE USES

1. Automated and High-Throughput Immunoblotting: Can be integrated into automated workflows for large-scale immunoblot screening for autoimmune diseases (e.g. Systemic Sclerosis) .

2. Quality Control of Complex Biologics: Used in Western blot analysis for purity, identity, and stability testing of monoclonal antibodies, fusion proteins, and viral vectors used in gene therapy .

3. Characterization of Protein Aggregates: Supports the transfer of proteins separated under non-denaturing conditions for detection of soluble and insoluble protein aggregates in neurodegenerative disease research (e.g., Tau, alpha-synuclein) .

4. Phosphoproteomics and Post-Translational Modification Analysis: Used for transferring proteins for probing with specific antibodies against phosphorylation, acetylation, and ubiquitination sites in cancer signaling research .

5. Process Analytical Technology (PAT) in Biomanufacturing: Used in pilot plants for in-process testing of harvest and purification steps to ensure product quality .

6. Cardiovascular Disease Biomarker Discovery: Supports large-scale validation studies of candidate protein biomarkers for coronary artery disease and heart failure .

7. Plant Pathology and Agricultural Research: Used for the detection of plant viral proteins and host-response proteins in GM crop research .

8. Neurodegenerative Disease Confirmation: Used as a confirmatory test for detecting pathological protein conformers (e.g., abnormal prion protein, phosphorylated tau) in cerebrospinal fluid (CSF) .

9. Detection of Rare Protein Variants in Genetic Screening: Supports the transfer of proteins for immunodetection in research on monogenic disorders affecting protein expression .

10. Veterinary Diagnostic Confirmatory Testing: Used in reference labs for confirmatory Western blot testing for diseases like Equine Infectious Anemia (EIA) and Feline Immunodeficiency Virus (FIV) .

11. Environmental and Food Allergen Testing: Used for the high-sensitivity detection of trace food allergens (e.g., peanut, gluten) in processed food products .

12. Clinical Assay Validation: Used in the validation and verification of new commercial Western blot kits for a chosen clinical marker .

1. BASIC IDENTIFICATION ATTRIBUTES

Device Type: A high-capacity, large-format wet transfer system for the electroblotting (electrotransfer) of proteins and nucleic acids from gels to membranes for Western, Southern, and Northern blotting in high-throughput clinical and research applications .

Designation: DL-ZY03 Transfer Electrophoresis Tank, High-Throughput Electroblotting System, Large-Format Western Blot Transfer Cell, DLAB DL-ZY03, High-Capacity Membrane Transfer Tank .

Key Components:

Tank Body: Large-format, highly transparent polycarbonate tank for real-time monitoring .

Electrode Core: 99.99% pure platinum wire electrodes for optimal conductivity and durability .

Dual Transfer Cassettes: Two independent cassettes, each capable of holding up to two mini-gels, enabling simultaneous processing of four blots .

Gel Holder Trays: User-friendly, open-ended trays for easy assembly of the gel-membrane sandwich .

Sponge Pads: High-quality foam pads (included) to ensure uniform pressure on the gel-membrane "sandwich" for even transfer .

Cooling System: An integrated, large-capacity ice box and two blue cooling units (included) for superior thermal management .

Bubble Roller: A special roller tool included to remove air bubbles from the gel-membrane sandwich before transfer .

2. TECHNICAL & PERFORMANCE PROPERTIES

Transfer Area per Cassette: 110 × 90 mm .

Gel Compatibility: Compatible with both mini-gels (83 × 73 mm) and large-format gels .

Maximum Capacity: Capable of transferring up to 4 mini-gels (83 × 73 mm) or 2 large gels per run .

Electrode Material: 99.99% high-purity platinum (Pt) wire, φ0.25mm, for excellent conductivity and corrosion resistance .

Electrical Design: Generates a uniform, high-strength electric field across the tank for efficient, consistent transfer .

Construction Material: Highly transparent, durable, and chemically resistant polycarbonate (PC) .

Safety: Incorporates design features to prevent incorrect polarity connection .

Dimensions (W×D×H): 270 × 120 × 160 mm (approx.) .

Weight: Approximately 1.7 kg .

3. PHYSICAL & OPERATIONAL PROPERTIES

Construction: Robust, leak-proof design manufactured via high-precision injection molding .

Electrode Quality: 99.99% high-purity platinum electrodes offer superior conductivity, corrosion resistance, and anti-aging properties .

Thermal Management: Features a large built-in ice reservoir and includes two blue cooling units, effectively absorbing heat generated during high-current runs .

Safety Design: Designed with a "limit function" on the upper cover to prevent the polarity from being reversed when connecting to the power supply .

High-Throughput Design: Dual independent transfer cassettes allow for streamlined workflow; one cassette can be loaded while another is transferring .

Compatibility: Designed to work seamlessly with standard mini-gel and large-format electrophoresis systems .

User Experience: Open-ended gel tray design and included bubble roller simplify the assembly of the gel-membrane sandwich, ensuring high-quality, reproducible transfers .

4. SAFETY & COMPLIANCE ATTRIBUTES

Regulatory Status: Medical laboratory equipment for in vitro diagnostic (IVD) use .

Safe Operation: Safety interconnects and cover design prevent operation if not correctly assembled.

Electrical Safety: Designed for safe low-voltage operation when used with a standard electrophoresis power supply .

Material Safety: High-strength, non-toxic polycarbonate material for the tank body .

5. STORAGE & HANDLING ATTRIBUTES

Storage: Store on a clean, dry benchtop. Ensure the tank is clean and dry before storage .

Cleaning: Rinse thoroughly with distilled water after each use. The polycarbonate tank is resistant to common buffers, but avoid harsh organic solvents .

Cooling Unit Care: Freeze the built-in ice box and two blue cooling units thoroughly before each use. Clean and dry after use to prevent mold growth .

Sponge Pad Care: Inspect and rinse sponge pads thoroughly with distilled water after each use to remove salt deposits. Squeeze dry and replace if they become compressed or torn .

Platinum Electrode Care: Handle with extreme care to prevent bending or physical damage. Clean by gentle rinsing with distilled water; never scratch the metal surface .

6. LABORATORY & CLINICAL APPLICATIONS

Primary Application: High-throughput wet electroblotting (electrotransfer) of proteins and nucleic acids for Western blotting, Southern blotting, Northern blotting, and other immunodetection methods in clinical diagnostic, pharmaceutical, and research laboratories .

Clinical Diagnosis: Increased throughput for confirmatory diagnostic testing for infectious diseases (HIV, Hepatitis, Lyme disease), autoimmune disorders, and genetic conditions via immunoblotting and nucleic acid hybridization .

1. SAFETY PRECAUTIONS

Correct Polarity: Ensure the transfer cassettes are correctly oriented with the tank's electrode core. The cover features a safety design to prevent incorrect polarity, but always double-check .

Buffer Level: Fill the tank completely with the recommended volume of chilled transfer buffer to fully submerge the transfer cassettes .

Temperature Control: Mandatory use of the pre-frozen ice box and cooling units is critical for high-intensity runs to prevent protein degradation and membrane damage .

Air Bubble Removal: Always use the supplied bubble roller to gently remove all air bubbles between the gel and the membrane. Trapped air bubbles create transfer voids, leading to inaccurate band patterns .

Membrane Handling: Use clean gloves and flat-tipped forceps when handling membranes to prevent contamination .

Electrical Safety: Do not open the lid or touch the buffer while the system is active to avoid electric shock.

Training: Only trained laboratory personnel should operate the DL-ZY03 for clinical diagnostic applications.

2. FIRST AID MEASURES

Buffer Spill: If Transfer Buffer (containing methanol and Tris-glycine) spills on skin, wash off immediately with soap and water . For eye exposure, flush with water for 15 minutes and seek medical attention.

Electrical Shock: Disconnect the power supply immediately and seek medical attention if any shock is received or suspected .