Mini Pipette Fixed Volume Pipette
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The Mini Pipette Fixed Volume Pipette from DLAB is a compact, single-channel mechanical pipette pre-set to a specific, non-adjustable volume for rapid, high-precision repetitive liquid handling. Featuring an ultra-light, one-piece molded body (~130 mm in length), it reduces user fatigue during high-throughput workflows. Integrated color-coding allows for immediate visual identification of the fixed volume (e.g., blue for 100 µL, orange for 50 µL), dramatically reducing selection errors in busy laboratories.
The pipette is fully autoclavable, ensuring sterility for aseptic cell culture and microbiology applications. Its universal tip cone is compatible with standard 200 μL and 300 μL tips. Each Mini Pipette is individually tested and supplied with an ISO 8655 calibration certificate, guaranteeing high precision and accuracy for standardized protocols.
For the medical laboratory technician, the fixed-volume design eliminates operator error and delivers fast, reproducible results for high-volume tasks like ELISA plate coating, PCR master mix preparation, and standardized sample inoculation for AST. For the patient, this reproducible sample handling ensures that diagnostic results for infectious diseases, antimicrobial susceptibility, and clinical chemistry are consistent, accurate, and reported with minimal turnaround time, directly supporting timely and effective therapeutic decisions.
Categories: LABORATORY EQUIPMENT AND SUPPLIES, Pipettes, Dispensers, and Lab Glassware
Tags: Air-Displacement Pipette, Autoclavable Pipette, Cell Culture, Clinical Diagnostics, DLAB Pipette, ELISA, Fixed Volume Pipette, ISO 8655, Laboratory Pipette, Microbiology, Micropipette, Mini Pipette, PCR, Single-Channel Pipette
Description
Mini Pipette Fixed Volume Pipette
PRIMARY CLINICAL & DIAGNOSTIC USES
1. Rapid Bulk Reagent Dispensing for High-Throughput Assays
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Primary Use: Provides a single-channel, spring-loaded mechanical pipette pre-set to a fixed volume, enabling rapid, consistent, and error-free dispensing of assay buffers, wash solutions, and bulk reagents for high-throughput ELISA and automated clinical chemistry platforms.
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How it helps: For the medical laboratory technician, the fixed-volume design eliminates the risk of operator-induced volume changes, ensuring each well receives exactly the intended reagent volume, thereby improving inter-assay precision. The fast, one-step operation allows for rapid dispensing into large numbers of tubes or plates, dramatically increasing throughput. For the patient, precise, high-speed reagent dispensing reduces well-to-well variability in diagnostic tests like ELISA, leading to more accurate detection of autoantibodies or infectious disease markers.
2. Fixed-Volume Sample Transfer for Standardized Microbiology
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Primary Use: Utilized for the precise, pre-determined delivery of bacterial suspensions, standardized inocula, and reagent volumes in antimicrobial susceptibility testing (AST) and colony counting procedures.
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How it helps: For the clinical microbiologist, the pipette’s fixed volume delivers an exact amount of sample every time, which is critical for performing standardized Kirby-Bauer disk diffusion tests or preparing McFarland standards. This consistency allows for accurate comparison of results across different batches and laboratories. For the patient, standardized and reproducible sample inoculation ensures that antibiotic susceptibility results (SIR – Susceptible, Intermediate, Resistant) accurately guide appropriate antibiotic therapy, improving treatment outcomes and reducing the risk of antimicrobial resistance.
3. Consistent Media and Buffer Preparation in Cell Culture
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Primary Use: Employed for the repeated, precise addition of culture media, trypsin, or dissociation reagents to multi-well plates, culture flasks, and microplates during routine cell culture and passaging procedures.
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How it helps: For the cell culture scientist, the fixed-volume design ensures that each culture vessel receives the same volume of nutrient medium or reagent, promoting uniform, consistent cell growth across all vessels and eliminating one potential variable in the experiment. The fully autoclavable tip cone and durable construction make it suitable for aseptic work within a biosafety cabinet. For the patient, consistent cell culture conditions support accurate outcomes for cell-based diagnostic tests and therapies, such as viral load testing for HIV or CMV, or the safety of cell-based products.
4. Standardized Reagent Addition for Qualitative Rapid Tests
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Primary Use: Used for the accurate addition of a fixed volume of sample or chase buffer to the sample well of lateral flow immunoassay devices (e.g., point-of-care test cassettes).
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How it helps: For the laboratory technician, the single fixed-volume step simplifies the process for running large batches of qualitative rapid tests. The user simply draws up the sample and dispenses the entire pre-set volume into the test cassette, reducing the chance of technique-based errors and improving consistency across operators. For the patient, standardized sample volume ensures the test strip develops correctly, providing clear, accurate positive/negative calls for a wide range of rapid infectious disease tests.
5. Color-Coded Design for Rapid Volume Identification
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Primary Use: Color-coded body markings (e.g., blue for 100 µL, orange for 50 µL, green for 10 µL) allow for immediate visual identification of the pipette’s fixed volume capacity, minimizing the risk of selecting the wrong pipette in a busy lab.
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How it helps: For the laboratory manager, this intuitive visual system reduces the time technicians spend verifying pipettes and helps prevent the use of an incorrect volume in a critical step. The compact, lightweight design and small size (~130 mm in length) also make the pipette easy to handle, store, and sterilize. For the patient, this intuitive color-coding directly reduces the risk of pre-analytical volume errors, which can lead to a failed batch of patient tests and costly, time-consuming repeats.
SECONDARY & SUPPORTIVE USES
1. ELISA plate coating – Provides consistent, fixed-volume delivery of capture antibodies or antigens to each well of a 96-well microplate during the plate coating process.
2. PCR and qPCR master mix preparation – Delivers a fixed, pre-determined volume of PCR master mix or primers to reaction tubes or plates, ensuring reaction consistency.
3. Blood grouping and serology – Transfers a fixed volume of patient blood or antisera (for ABO/Rh typing) to test cards or tubes for compatibility testing in blood banks.
4. Hematology sample preparation – Adds a fixed volume of whole blood or diluent for manual cell counting using a hemocytometer or for preparing blood smears.
5. Preparation of quality control (QC) samples – Accurately delivers a fixed volume of analyte stock solution to tubes for preparation of standard curves or QC samples for HPLC analysis.
6. Urinalysis and chemical reagent addition – Adds a fixed volume of chemical reagents (e.g., sulfosalicylic acid) to urine samples for protein precipitation and turbidity tests.
7. Preparation of protein assay standards – Pipettes a fixed volume of BSA standard solution for constructing standard curves for Lowry, Bradford, or BCA protein assays.
8. Addition of staining solutions in histology – Dispenses a fixed volume of hematoxylin or eosin stain onto microscope slides for routine histological staining.
9. Standardized antibody addition in immunofluorescence – Delivers a fixed volume of primary or secondary antibody solution to wells for cell staining assays.
10. Application of dental materials – Used for the precise placement of dental bonding agents, cements, or etchants during laboratory work or clinical material preparation.
11. Addition of fixing solutions in cytology – Dispenses a fixed volume of cytological fixative to preserve cell morphology on slides for Pap smear screening.
KEY PRODUCT FEATURES
1. BASIC IDENTIFICATION ATTRIBUTES
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Device Type: A fully autoclavable, single-channel mechanical air-displacement pipette with a pre-set, non-adjustable fixed volume, offering a compact, lightweight design ideal for high-frequency, repetitive liquid handling in clinical and molecular laboratories.
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Designation: Micro Pipette, Fixed Volume Pipette, DLAB Fixed Volume Pipette, Mini Pipette Standard Model, Color-Coded Fixed Pipette.
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Key Components:
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Volume Fixed – Factory-set non-adjustable volume (e.g., 10 µL, 25 µL, 50 µL, 75 µL, 100 µL, 200 µL).
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Color Coding – Distinct body colors corresponding to different fixed volumes (e.g., blue: 100 µL, orange: 50 µL, green-light: 10 µL) for immediate visual identification.
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One-Piece Molded Body – Compact, highly durable, and lightweight (approx. 130 mm total body length).
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Universal Tip Cone – Compatible with standard 200 μL and 300 μL universal pipette tips.
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Tip Ejector – Integrated, easy-to-press button for quick, hygienic tip ejection.
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Material – Corrosion-resistant, durable polymers with excellent chemical resistance.
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2. TECHNICAL & PERFORMANCE PROPERTIES
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Pre-Set Fixed Volumes: 5 µL, 10 µL, 20 µL, 25 µL, 50 µL, 75 µL, 100 µL, 200 µL, 250 µL, 300 µL, 500 µL, 1000 µL.
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Number of Channels: 1.
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Sterilization: Entire pipette (except the interior mechanism) is fully autoclavable at 121 °C and 1 atm.
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Operating Principle: Spring-loaded mechanical air-displacement piston system.
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Tip Compatibility: Designed for use with standard 200 μL universal tips and 300 μL tips.
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Length: Ultra-short design, approximately 130 mm.
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Internal Components: High-performance, chemically resistant piston and seals.
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Regulatory Compliance: Complies with ISO 8655/DIN 12650 volumetric standards for piston-operated pipettes.
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Quality Standards: Manufactured under ISO 9001, ISO 13485, and GLP standards.
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Color Coding for Volumes: Volumes are color-coded for different volume ranges, such as 25-300 µL for high-end models, with different colors for different volumes to facilitate easy choosing.
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Customization: Volumes from 5 µl to 5000 µl available.
3. PHYSICAL & OPERATIONAL PROPERTIES
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Construction: Robust, one-piece molded, ultra-light, and compact polypropylene body.
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Operation: Simple, single-handed operation with a short piston stroke and low activation force.
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Volume Display: Volume is not adjustable; the capacity is permanently marked on the body and color-coded.
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Tip Ejection: Easy, fatigue-free tip ejection mechanism.
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Sterilization: Autoclavable parts are compatible with standard steam sterilization cycles.
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Portability: Compact, lightweight design for convenient benchtop use and storage.
4. SAFETY & COMPLIANCE ATTRIBUTES
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Regulatory Status: General laboratory equipment for in vitro diagnostic (IVD) use.
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Sterilization Safety: Autoclavability guarantees sterility for handling biohazardous samples and cell culture.
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Chemical Resistance: High chemical resistance of all components protects against damage from acids, bases, and organic solvents, ensuring long durability.
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Quality Compliance: Compliant with ISO 8655 and comes with an individual calibration certificate.
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Tip Seal: The universal tip cone ensures a secure, airtight seal and accurate volume delivery.
5. STORAGE & HANDLING ATTRIBUTES
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Storage: Store upright in a pipette stand to protect the tip cone from contamination.
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Cleaning: Wipe exterior surfaces with 70% isopropyl alcohol.
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Autoclaving: The entire pipette can be autoclaved (refer to manufacturer's guidelines).
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Inspection: Check for damage and ensure the tip cone is clean before each use.
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Operation – Tip Attachment: Press the pipette vertically onto a universal tip until a firm seal is felt.
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Operation – Aspiration: Depress the plunger smoothly to the first stop, immerse the tip below the liquid surface, and slowly release the plunger.
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Operation – Dispensing: Place the tip against the receiving vessel wall, depress the plunger to the first stop to dispense, and then to the second stop to expel any residual liquid.
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Calibration: Recalibration is performed by the manufacturer; user-calibration is not applicable due to fixed volume.
6. LABORATORY & CLINICAL APPLICATIONS
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Primary Application: Highly precise, error-free, and repeatable addition of bulk reagents, samples, and standardized inocula for high-throughput molecular diagnostics, microbiology AST, clinical chemistry, and cell culture.
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Clinical Role: An essential instrument for clinical diagnostic, microbiology, research, and quality control laboratories that require fast, consistent, and accurate liquid handling for standardized patient sample testing.
SAFETY HANDLING PRECAUTIONS
1. SAFETY PRECAUTIONS
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Tip Fit: Ensure the universal tip is securely attached before use to prevent leakage.
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Correct Pipette: Verify the color-coding or label to ensure the correct fixed-volume pipette is selected for the protocol.
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Avoid Contamination: Use a new, sterile tip for each sample to prevent cross-contamination.
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Cleaning: Do not immerse the entire pipette in liquid unless it is fully disassembled.
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Training: All users must be trained in proper liquid handling techniques and volume verification.
2. FIRST AID MEASURES
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Chemical Contamination: If the pipette is contaminated with a hazardous chemical, refer to the chemical's Safety Data Sheet (SDS) for specific decontamination and first aid instructions.
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Mechanical Injury: If cut by a broken tip or glass, wash the wound, apply pressure, and seek medical attention.
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Autoclave Burn: Allow the pipette to cool completely after autoclaving before handling.
3. FIRE FIGHTING MEASURES
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Flammability: Polypropylene and other plastic components are combustible.
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Extinguishing Media: Use carbon dioxide (CO₂), dry chemical fire extinguisher, or alcohol-resistant foam for fires involving plastic materials.
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