Multi-functional Horizontal Electrophoresis Tank (DL-SUB02)

Multi-functional Horizontal Electrophoresis Tank (DL-SUB02)

PRIMARY CLINICAL & DIAGNOSTIC USES

1. High-Throughput PCR Product Confirmation for Infectious Disease Testing

• Primary Use: Provides a versatile horizontal electrophoresis tank with interchangeable gel trays (120 × 120 mm, 120 × 60 mm, 60 × 120 mm, 60 × 60 mm) and multiple comb configurations, enabling high-throughput separation of PCR products for infectious disease diagnosis .

• How it helps: For the molecular biologist and clinical laboratory scientist, the DL-SUB02 delivers flexible sample loading capacity from 5 to 2500 base pairs—allowing confirmation of up to 83 samples per run (using 3 rows of 8-well combs + 2 rows of 11-well combs). For the patient undergoing molecular diagnostic testing for COVID-19, influenza, hepatitis, HIV, or other infectious diseases, efficient high-throughput processing reduces turnaround time for test results, enabling earlier diagnosis and treatment initiation.

2. Restriction Fragment Analysis for Genetic Screening

• Primary Use: Supports restriction fragment length polymorphism (RFLP) analysis, genetic fingerprinting, and mutation detection using standard 1.0 mm thickness combs .

• How it helps: For the molecular geneticist and clinical laboratory scientist, the DL-SUB02’s 1.0 mm comb thickness provides optimal sample volume for restriction fragment analysis—enabling detection of genetic mutations associated with inherited disorders (e.g., sickle cell disease, cystic fibrosis, BRCA mutations). For the patient undergoing genetic screening, accurate fragment separation supports definitive diagnosis and informs clinical management decisions.

3. Nucleic Acid Quality Assessment for Molecular Diagnostics

• Primary Use: Used for rapid assessment of DNA and RNA integrity, purity, and concentration before downstream molecular applications such as PCR, sequencing, and hybridization assays .

• How it helps: For the clinical laboratory scientist, the DL-SUB02 provides visual confirmation of nucleic acid quality—enabling detection of degradation, contamination, or concentration issues that could compromise diagnostic test results. For the patient, proper sample quality assessment ensures that only high-quality specimens proceed to diagnostic testing, reducing the risk of false-negative or false-positive results.

4. Multiplex Pathogen Detection and Resolution

• Primary Use: Supports multiplex PCR product resolution using long gels (120 × 120 mm configuration) with sample loads up to 1500 bp, enabling simultaneous detection of multiple pathogen targets in a single lane .

• How it helps: For the clinical microbiologist, the DL-SUB02’s long gel configuration provides extended separation distance for resolving multiple PCR amplicons of different sizes—enabling multiplex detection of respiratory virus panels, gastrointestinal pathogen panels, and sexually transmitted infection panels . For the patient, simultaneous testing for multiple pathogens reduces the number of specimens required and accelerates diagnosis of co-infections.

SECONDARY & SUPPORTIVE USES

1. RNA Denaturing Gel Electrophoresis: Used for formaldehyde-agarose gel electrophoresis for assessing RNA integrity and purity (28S/18S ratio) in molecular diagnostics .

2. Gel Extraction and Purification: Used for separation and visualization of target DNA fragments before gel extraction for downstream applications (e.g., sequencing, cloning).

3. Southern Blotting Sample Preparation: Provides DNA separation for subsequent transfer to membranes for hybridization-based detection.

4. Microbial Pathogen Identification: Used for PCR product confirmation in bacterial, viral, and fungal identification workflows in clinical microbiology .

5. DNA Fragment Size Determination: Supports molecular weight determination of DNA fragments using DNA ladders and size markers.

6. Blood Bank and Transfusion Medicine: Supports DNA-based blood group genotyping and donor screening for blood-borne pathogens.

7. Food Safety and Quality Testing: Supports GMO detection, foodborne pathogen identification, and food authenticity testing.

8. PFGE Sample Confirmation: Used for checking DNA quality and size range before pulsed-field gel electrophoresis for bacterial typing in outbreak investigations.

9. Veterinary Diagnostic Laboratory: Used for PCR product confirmation and nucleic acid analysis for animal health testing .

10. Environmental Microbiology Testing: Used for detection of microbial pathogens in water, soil, and environmental samples by PCR and electrophoresis .

11. Pharmaceutical Quality Control: Used for nucleic acid purity assessment in biopharmaceutical manufacturing .

12. Pharmacogenomics Testing: Supports DNA fragment analysis for genotyping of drug metabolism genes (e.g., CYP450) .

13. Research and Academic Laboratories: Provides flexible electrophoresis for DNA fragment analysis in molecular biology research .

14. Educational and Training Laboratories: Supports teaching of PCR and electrophoresis techniques in medical and graduate training programs .

15. Method Development and Optimization: Used to optimize electrophoresis protocols for clinical implementation .

16. Troubleshooting and Quality Assurance: Supports investigation of PCR failures, contamination events, and assay performance issues .