UV-Vis Spectrophotometer (SP-UV1100)

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The UV-Vis Spectrophotometer (SP-UV1100) is a high-performance, single-beam benchtop instrument engineered for accurate, reproducible analysis of nucleic acids, proteins, clinical chemistry analytes, and pharmaceutical compounds in clinical diagnostics, molecular biology, pharmaceutical quality control, and research laboratories. Covering a wavelength range of 200–1000 nm with automatic switching between tungsten and deuterium lamps, this spectrophotometer provides the analytical versatility required for routine and specialized laboratory workflows, including nucleic acid purity assessment, enzyme kinetics, and quantitative clinical chemistry.

Every UV-Vis Spectrophotometer (SP-UV1100) is individually factory calibrated and supplied with a calibration certificate traceable to national standards, delivering exceptional photometric accuracy of ±0.003 A at 0.5 A and photometric repeatability of ≤0.0015 A at 0.5 A. The instrument’s baseline stability of ≤0.002 A/h at 500 nm after a 2-hour warm-up ensures reliable, drift-free measurements for endpoint and kinetic assays. A large 128 × 64 dot backlit LCD display with adjustable brightness provides clear data visualization, and the 4-position sample compartment accommodates standard 10 mm path length cuvettes, with optional holders available for path lengths from 5 to 100 mm.

Key user-focused features include automatic wavelength calibration via arrow keys to minimize user errors, large-capacity data storage for up to 200 test data groups and 200 standard curves with automatic backup to prevent data loss during power interruption, and multi-function measurement modes including photometric (fixed wavelength), quantitative analysis (standard curve), and multi-wavelength scanning (up to 10 wavelengths). The optional Wave Professional PC software enables advanced functions including spectrum scanning, time-scan kinetics (up to 12 hours), and specialized nucleic acid/protein measurement functions for molecular biology applications.

The UV-Vis Spectrophotometer (SP-UV1100) is supplied with four glass and two quartz square cuvettes (3.5 mL, 10 mm path length) and includes USB and parallel ports for printer connection and data export. The instrument is manufactured under ISO 9001:2015 and ISO 13485:2016 quality management systems, and the manufacturer’s CNAS-certified calibration laboratory ensures measurement traceability and confidence in results.

For the clinical laboratory scientist, molecular biologist, and pharmaceutical quality control analyst, the UV-Vis Spectrophotometer (SP-UV1100) delivers the accuracy, stability, and versatility required for DNA/RNA purity assessment (260/280 and 260/230 ratios), protein quantification (A280, Bradford, BCA, Lowry), enzyme kinetic assays (ALT, AST, ALP, GGT, LDH, CK), and routine clinical chemistry testing (glucose, cholesterol, triglycerides, bilirubin, creatinine, uric acid). For the patient, reliable, traceable spectrophotometric analysis ensures that diagnostic results are accurate and reproducible, directly supporting effective clinical decision-making, appropriate treatment selection, and confident disease monitoring.

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Description

UV-Vis Spectrophotometer (SP-UV1100)

PRIMARY CLINICAL & DIAGNOSTIC USES

1. High-Precision Nucleic Acid Quantification and Purity Assessment for Molecular Diagnostics
Primary Use: Provides a single-beam UV-Vis spectrophotometer with a dual light source (tungsten and deuterium lamps) covering a wavelength range of 200–1000 nm, used for the accurate quantification and purity assessment of DNA, RNA, and oligonucleotides in clinical molecular diagnostics, including infectious disease testing, oncology biomarkers, and genetic screening workflows.

How it helps: For the molecular diagnostics scientist, the UV-Vis Spectrophotometer (SP-UV1100) measures absorbance at 260 nm for nucleic acid concentration and automatically calculates 260/280 and 260/230 purity ratios, ensuring that extracted DNA or RNA meets stringent quality standards for downstream PCR, qPCR, or next-generation sequencing. The instrument’s photometric accuracy of ±0.003 A at 0.5 A and stability of ≤0.002 A/h deliver consistent, reliable results. For the patient, precise nucleic acid quantification and purity confirmation directly support accurate detection of viral load (HIV, hepatitis, COVID-19), cancer-associated mutations, and inherited genetic disorders, reducing the risk of false negatives caused by contaminated or degraded samples.

2. Protein Concentration Determination for Clinical Biochemistry and Immunology
Primary Use: Enables direct measurement of protein concentration via absorbance at 280 nm (A280 method) for purified proteins, antibodies, and serum samples, as well as colorimetric protein assays including Bradford, BCA, and Lowry using visible wavelength detection, with quantitative analysis mode using standard curves.

How it helps: For the clinical biochemistry technologist and immunology specialist, the SP-UV1100 provides rapid, accurate protein quantification for patient serum, cerebrospinal fluid, urine, and monoclonal antibody preparations. The instrument’s photometric range of -0.3 to 3.0 A accommodates a wide range of clinical sample concentrations, and the large LCD display allows easy data review. For the patient, accurate protein quantification supports diagnosis and monitoring of conditions such as nephrotic syndrome, liver disease, multiple myeloma, and nutritional disorders, as well as therapeutic antibody dosing, enabling timely clinical interventions.

3. Enzymatic Activity and Kinetic Monitoring for Clinical Enzyme Assays
Primary Use: Performs time-scan (kinetics) measurements for monitoring enzymatic reactions over time, used for the clinical determination of enzyme activities including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), and creatine kinase (CK) in patient serum samples.

How it helps: For the clinical chemistry laboratory scientist, the SP-UV1100’s kinetics mode tracks absorbance changes at specific wavelengths over time, allowing calculation of enzyme reaction rates in units per liter (U/L). The instrument’s stability of ≤0.002 A/h after warm-up ensures consistent, drift-free kinetic measurements essential for accurate enzyme activity calculation. For the patient undergoing liver function testing, cardiac enzyme panels (troponin, CK-MB), or metabolic disorder screening, accurate enzyme activity results directly guide diagnosis of hepatitis, myocardial infarction, muscular dystrophy, and other conditions, as well as monitoring of disease progression and treatment response.

4. Quantitative Analysis of Blood and Serum Components for Routine Clinical Chemistry
Primary Use: Used for the quantitative analysis of various blood and serum components, including glucose, cholesterol, triglycerides, bilirubin (total and direct), creatinine, uric acid, and electrolytes, using endpoint measurement modes with standard curve quantification based on the Beer-Lambert law.

How it helps: For the clinical chemistry technologist, the UV-Vis Spectrophotometer (SP-UV1100) measures the concentration of key metabolites by detecting the absorbance of colored reaction products generated by commercial clinical chemistry reagent kits. The instrument’s 4-position sample compartment allows sequential processing of multiple samples, and the large-capacity data storage for up to 200 standard curves reduces repetitive calibration. For the patient, reliable measurements of blood glucose, lipid panels, kidney function markers (creatinine, BUN), and liver enzymes (bilirubin) support routine health screening, chronic disease management (diabetes, hyperlipidemia, chronic kidney disease), and acute care diagnostics directly at the point of sample processing.

SECONDARY & SUPPORTIVE USES

1. Urinalysis parameter quantification: Measures colorimetric reactions for urine test strips and liquid reagents, quantifying parameters including protein, glucose, ketones, bilirubin, urobilinogen, nitrite, leukocyte esterase, and occult blood for routine urinalysis in clinical and research laboratories.

2. Therapeutic drug monitoring: Quantifies concentrations of pharmaceutical drugs in patient serum or plasma for drugs with narrow therapeutic windows, including antiepileptics (phenytoin, valproic acid), immunosuppressants (cyclosporine, tacrolimus), and certain antibiotics (vancomycin, gentamicin), using validated UV-visible absorption methods.

3. Cell density measurement for clinical microbiology: Measures optical density (OD) at 600 nm (OD600) of bacterial and yeast cultures to monitor microbial growth, determine cell concentration, and optimize harvest timing in clinical microbiology laboratories for susceptibility testing and vaccine production.

4. Hemoglobin and bilirubin measurement in neonatal screening: Quantifies total and direct bilirubin levels in neonatal serum or transcutaneous samples for jaundice assessment, and measures hemoglobin for anemia screening in newborn and pediatric populations, supporting early intervention for hyperbilirubinemia.

5. Immunoassay endpoint detection (ELISA): Serves as the detection platform for various clinical immunoassays including ELISA, where the spectrophotometer measures the final colored product absorbance (e.g., at 450 nm, 492 nm, or 650 nm) to quantify antibody or antigen concentrations in patient samples for infectious disease serology, autoimmune testing, and allergy screening.

6. Drug dissolution testing for pharmaceutical quality control: Quantifies drug release from solid dosage forms (tablets, capsules) in dissolution testing, measuring the concentration of active pharmaceutical ingredient in dissolution media over time for quality control release testing and stability studies.

7.Water quality testing for clinical utilities: Monitors the quality of purified water used in clinical laboratories, dialysis centers, and pharmacy compounding areas by measuring parameters such as chlorine, nitrate, nitrite, and total organic carbon, ensuring compliance with clinical water standards.

8. Veterinary diagnostic sample analysis: Performs the same range of clinical chemistry, protein quantification, and nucleic acid purity assessments for veterinary patient samples (blood, serum, urine, tissue homogenates) in animal health diagnostic laboratories and veterinary teaching hospitals.

9. Forensic toxicology screening: Quantifies drugs of abuse (e.g., amphetamines, benzodiazepines, cannabinoids), alcohol markers (ethanol, ethyl glucuronide), and certain poisons (cyanide, carbon monoxide) in biological specimens (blood, urine, gastric contents) for forensic casework using validated UV-visible spectrophotometric methods.

10. Point-of-care test validation in research settings: Serves as a reference method for validating new point-of-care testing devices, rapid diagnostic tests, and laboratory-developed assays during research and development, providing gold-standard spectrophotometric comparison data.

11. Food safety and nutritional analysis: Analyzes food and beverage samples for components such as caffeine, total polyphenols, vitamin C (ascorbic acid), nitrites/nitrates, and food colorants in food safety testing, nutritional labeling, and quality control laboratories.

12. Pharmaceutical raw material testing: Quantifies the concentration of active pharmaceutical ingredients and excipients during incoming raw material quality control, ensuring that pharmaceutical ingredients meet specification before use in drug manufacturing, as required by Good Manufacturing Practices (GMP).

KEY PRODUCT FEATURES

1. BASIC IDENTIFICATION ATTRIBUTES

Device Type: A single-beam, scanning UV-Vis spectrophotometer with dual-light-source configuration (tungsten and deuterium lamps) for routine laboratory analysis.
Designation: SP-UV1100 UV-Vis Spectrophotometer, DLAB SP-UV1100, Scilogex SP-UV1100.
Key Components:
- Tungsten lamp (visible range, 320–1000 nm) and deuterium lamp (UV range, 200–320 nm) with automatic switching
- 128 × 64 dot backlit LCD display with adjustable brightness and graphical display capabilities
- 4-position sample compartment for 10 mm path length cuvettes, with optional holders for 5–100 mm path lengths
- USB port and parallel port for printer connection
- Data storage RAM for up to 200 test data groups and 200 standard curves, with automatic backup
- Optional Wave Professional PC software for computer control and advanced analysis functions

2. TECHNICAL & PERFORMANCE PROPERTIES

Optical system: Single beam with dual light source (tungsten and deuterium lamps) with automatic switching based on wavelength
Spectral bandwidth: 4 nm
Wavelength range: 200–1000 nm (standard model)
Wavelength accuracy: ±2 nm
Wavelength repeatability: ≤1 nm
Photometric range: -0.3 to 3.0 A (absorbance) and 0 to 200% T (transmittance)
Photometric accuracy: ±0.003 A at 0.5 A; ±0.006 A at 1.0 A; ±0.5% T at 100% T
Photometric repeatability: ≤0.0015 A at 0.5 A; ≤0.003 A at 1.0 A; ≤0.25% T at 100% T
Stability (baseline drift): ≤0.002 A/h at 500 nm after 2-hour warm-up
Stray light: ≤0.2% T at 220 nm (NaI) and 360 nm (NaNO₂)
Sample compartment: 4-position cuvette holder, standard for 10 mm path length square cuvettes (10 × 10 × 45 mm, 3.5 mL). Optional holders available for 5, 10, 20, 30, 40, 50, and 100 mm path lengths
Data storage capacity: Up to 200 test data groups and 200 standard curves stored in RAM; data retained after power failure (non-volatile)
Wavelength calibration: Automatic wavelength setting with arrow keys for easy calibration and reduced user errors
Output interfaces: USB port for PC connection and data export; parallel port (IEEE 1284) for direct printer connection
Power requirements: 110/220 VAC ±10%, 50/60 Hz, 120 W (user-selectable)
Dimensions (L × W × H): 490 × 360 × 210 mm
Weight: Approximately 12 kg
Quality compliance: Manufactured under ISO 9001 and ISO 13485 quality management systems; CNAS-certified calibration laboratory ensures measurement traceability

3. PHYSICAL & OPERATIONAL PROPERTIES

Construction: Durable, chemical-resistant benchtop enclosure with stable, vibration-dampening base for precise optical measurements
Display: Large 128 × 64 dot backlit LCD screen with adjustable brightness for clear data visualization, including graphic display of spectra and time scans
Operation: Fully standalone operation with all measurement functions executable directly from the instrument; no PC required for routine use
Sample loading: 4-position cuvette holder allows up to four samples to be queued for sequential measurement, improving workflow efficiency
Measurement modes: Photometric mode (fixed wavelength single-point measurement), quantitative analysis mode (standard curve based on up to 9 standards), multi-wavelength scanning mode (up to 10 wavelengths), and time-scan kinetics mode (requires optional software)
Data management: Large-capacity internal data storage for up to 200 test results and 200 standard curves; automatic data backup protects against loss during power interruption; data can be exported via USB
Portability: Benchtop unit weighing 12 kg; not portable but occupies standard laboratory benchtop footprint
Software compatibility: Optional Wave Professional software enables computer control, spectrum scanning (190–1100 nm), time scanning (kinetics up to 12 hours), multi-wavelength scanning (up to 10 wavelengths), and specialized nucleic acid/protein measurement functions
Maintenance: Tungsten and deuterium lamps are user-replaceable; automatic lamp switching optimizes lamp life by using the appropriate lamp for the selected wavelength range
Included accessories: 4 glass square cuvettes (3.5 mL, for visible range >340 nm) and 2 quartz square cuvettes (3.5 mL, for UV range <340 nm), power cord, dust cover, operating manual, and calibration certificate

4. SAFETY & COMPLIANCE ATTRIBUTES

Regulatory Status: General laboratory equipment for in vitro diagnostic (IVD) use; manufactured under ISO 9001:2015 and ISO 13485:2016 quality management systems; CNAS-certified calibration laboratory ensures measurement traceability to national standards
UV Radiation Safety: Deuterium lamp emits UV radiation. Sample compartment cover must remain closed during operation. Interlock mechanism prevents operation with cover open on compliant models
Lamp Safety: Tungsten and deuterium lamps operate at high temperatures (up to 200°C); allow at least 30 minutes cooling before handling or replacement
Electrical Safety: Compliant with IEC 61010-1 for laboratory electrical equipment; grounded power cord; built-in fuse and overload protection
Chemical Safety: Sample compartment and cuvette holder are designed to contain minor spills; exterior surfaces are chemical-resistant to common laboratory reagents (alcohols, dilute acids, dilute bases)
Data Integrity: Automatic backup to non-volatile RAM prevents data loss during power interruption; password-protected calibration settings available (with optional software)
Quality Compliance: Each instrument undergoes individual factory testing; supplied with a calibration certificate traceable to international standards; recommended annual recalibration

5. STORAGE & HANDLING ATTRIBUTES

Storage: Store in a clean, dry, dust-free environment at 15–30°C and ≤80% relative humidity (non-condensing). Cover instrument with dust cover when not in use to protect optical components. Avoid storage near strong magnetic fields, heat sources, or direct sunlight
Installation: Place on a stable, vibration-free benchtop or table away from air vents, windows, and high-traffic areas. Allow at least 10 cm clearance on all sides for ventilation. Do not place near centrifuges or vortex mixers that cause vibration
Warm-up procedure: Turn on instrument and allow 2-hour warm-up before performing critical clinical measurements to achieve specified stability of ≤0.002 A/h. For routine qualitative checks, 30-minute warm-up may be sufficient
Cleaning: Wipe exterior surfaces with a soft, lint-free cloth slightly dampened with 70% isopropyl alcohol or mild detergent solution. Do not spray liquids directly onto the instrument. Clean sample compartment after each use with a lint-free cloth to remove spills and dust. Do not use abrasive cleaners or organic solvents (acetone, chloroform) on the instrument body
Cuvette handling: Use only clean, scratch-free, optically transparent cuvettes. For UV measurements below 340 nm, quartz cuvettes are required. For visible measurements above 340 nm, glass cuvettes are acceptable. Handle cuvettes by the frosted sides or upper rim to avoid fingerprints on optical surfaces. Clean cuvettes immediately after use with appropriate solvent and dry with lint-free tissue
Lamp maintenance: Tungsten lamp lifetime approximately 2000 hours; deuterium lamp lifetime approximately 2000 hours. Replace lamps when displayed intensity falls below acceptable levels or at manufacturer-recommended intervals. Allow lamps to cool completely before replacement. Use only manufacturer-specified replacement lamps
Wavelength calibration: Perform automatic wavelength calibration at least weekly or before critical measurements using the auto-setting function accessible via the arrow keys. Use the built-in holmium oxide filter (if equipped) or standard holmium oxide solution for verification
Standard curve preparation: Prepare fresh calibration standards for quantitative analysis daily or as recommended by reagent kit instructions. Store up to 200 standard curves in instrument memory for repeated use; verify curve periodically with a known control standard
Software installation: To install optional Wave Professional PC software, contact manufacturer for compatibility and system requirements. Software enables advanced functions including spectrum scanning, kinetics analysis, and nucleic acid/protein measurement
Periodic verification: Perform photometric accuracy and wavelength accuracy verification monthly using certified reference materials or manufacturer-provided calibration filters. Document results for quality assurance records

6. LABORATORY & CLINICAL APPLICATIONS

Primary Application: Quantitative and qualitative UV-Vis spectrophotometric analysis of biomolecules (nucleic acids, proteins), clinical chemistry analytes (glucose, cholesterol, triglycerides, bilirubin, creatinine, uric acid, enzymes), pharmaceutical compounds, and environmental samples in clinical diagnostics, molecular biology, pharmaceutical quality control, and research laboratories.
Clinical Role: An essential instrument for clinical diagnostic laboratories, molecular diagnostics (MDx) labs, hospital clinical chemistry departments, immunology laboratories, blood banks (for plasma hemoglobin detection), pathology departments, pharmaceutical quality control facilities, forensic laboratories, and research institutions. Critical for patient sample analysis (DNA/RNA purity and concentration, protein quantification, enzyme activities, metabolite levels, therapeutic drug monitoring) where accurate, reproducible, and traceable spectrophotometric measurement is mandatory for diagnosis, disease monitoring, treatment guidance, and regulatory compliance.

SAFETY HANDLING PRECAUTIONS

1. SAFETY PRECAUTIONS

UV Radiation Hazard: The deuterium lamp emits intense UV radiation (200–320 nm). Always keep the sample compartment cover completely closed during operation. Never insert fingers or objects into the sample compartment while the instrument is powered on. Do not stare directly at the light source or open the deuterium lamp housing
Electrical Safety: Do not operate the instrument with wet hands. Disconnect the power cord before cleaning, servicing, or replacing lamps. Use only the supplied grounded power cord and ensure the outlet provides proper grounding. Do not operate if the power cord is damaged or if liquid has spilled into the instrument
Hot Surface Hazard: The tungsten lamp and deuterium lamp become extremely hot during operation (surface temperatures can exceed 150°C). Allow lamps to cool for at least 30 minutes before handling or replacement. Do not touch the lamp housing during or immediately after operation
Chemical Hazard: Handle all reagents, standards, control materials, and patient samples according to laboratory safety protocols and applicable Safety Data Sheets (SDS). Wear appropriate personal protective equipment (PPE) including nitrile gloves, laboratory coat, and safety glasses or face shield. Clean spills immediately to prevent damage to instrument optics and potential chemical exposure
Biohazard Hazard: Patient samples (serum, plasma, urine, cerebrospinal fluid, tissue homogenates) may contain infectious agents. Follow all institutional biosafety guidelines. Use leak-proof cuvettes for biohazardous samples. Decontaminate the sample compartment after each use with appropriate disinfectant (e.g., 10% bleach solution followed by water rinse, then 70% alcohol)
Cuvette Safety: Handle glass and quartz cuvettes with care; breakage can cause cuts from sharp glass shards. Discard broken cuvettes immediately in an appropriate sharps container. Do not use cuvettes with chips or cracks
Sample Containment: Ensure cuvettes are properly seated in the sample holder to prevent tipping or spillage. For volatile or hazardous samples, use sealed cuvettes with caps. Do not overfill cuvettes (maximum volume 3.5 mL for standard cuvettes, fill to approximately 2.5–3.0 mL)
Stability Requirement: Allow the instrument to warm up for 2 hours before performing critical clinical measurements to achieve specified stability of ≤0.002 A/h. Failure to warm up may result in inaccurate results due to baseline drift
Vibration Avoidance: Do not operate the instrument on the same benchtop as centrifuges, vortex mixers, or other vibration-generating equipment. Vibration can cause unstable readings and measurement errors
Training Requirement: Only trained laboratory personnel with appropriate technical background in spectrophotometry and clinical laboratory procedures should operate the SP-UV1100 for clinical diagnostic applications. Users should read and understand the operator's manual before first use

2. FIRST AID MEASURES

UV Exposure to Eyes: If the sample compartment cover is opened during operation or UV radiation exposure is suspected from the deuterium lamp, immediately close the cover. Do not rub eyes. Rest eyes and seek ophthalmologic evaluation if symptoms develop including eye pain, redness, photophobia (light sensitivity), tearing, or blurred vision. Report the exposure to the laboratory supervisor
UV Exposure to Skin: Prolonged UV exposure may cause skin erythema (redness resembling sunburn). Cool the affected area with cool running water for 15 minutes. If blistering or severe pain develops, seek medical attention
Burn from Hot Lamp: If contact with a hot lamp or lamp housing occurs, immediately cool the burn area with cool (not cold) running water for 15 minutes. Do not apply ice directly to the burn. Remove any jewelry or constrictive items from the affected area. Cover the burn with a sterile, non-adhesive dressing. Seek medical attention for burns covering a large area, deep burns, or burns causing blistering
Chemical Splash to Eyes: If a chemical sample, reagent, or cleaning solution splashes into the eyes, immediately flush eyes with copious amounts of clean water or sterile saline solution for at least 15 minutes. Hold eyelids open to ensure thorough rinsing. Remove contact lenses if present and easily removable. Seek immediate medical attention after flushing
Chemical Splash to Skin: Wash contaminated skin with soap and water thoroughly for at least 5 minutes. Remove contaminated clothing. If irritation, redness, or chemical burns develop, seek medical evaluation. Refer to the specific SDS for the chemical involved
Sharps Injury (Broken Cuvette): If broken cuvette glass punctures the skin, allow the wound to bleed freely (do not squeeze). Wash the wound thoroughly with soap and warm water for 15 minutes. Apply antiseptic and cover with sterile bandage. If the cuvette contained patient biological material, follow the facility's exposure management protocol for bloodborne pathogens (e.g., HIV, hepatitis B/C). Report the injury to the laboratory supervisor immediately
Inhalation of Chemical Vapors: If chemical vapors from volatile reagents (e.g., organic solvents) are inhaled during sample preparation, move to fresh air immediately. If breathing is difficult, administer oxygen if trained. Seek medical attention if coughing, dizziness, nausea, or respiratory distress persists
Electrical Shock: If an electrical shock incident occurs, disconnect power at the circuit breaker before attempting to assist the victim. Do not touch the victim until power is disconnected. Once safe, assess the victim for responsiveness and breathing. Call emergency medical services immediately. Perform CPR if trained and necessary. Seek medical evaluation for all electrical shock victims, even if symptoms appear mild

3. FIRE FIGHTING MEASURES

Flammability: The instrument's plastic housing (ABS/polycarbonate), internal cables, and electronic components are combustible and will burn if exposed to flame or high heat. Tungsten and deuterium lamps operate at high temperatures and can ignite nearby flammable materials. The power supply contains capacitors and other flammable components
Extinguishing Media: Use carbon dioxide (CO₂) or dry chemical (ABC) extinguisher for electrical fires involving the spectrophotometer. Do not use water directly on the instrument as water conducts electricity and can cause electrical shock or damage. For larger laboratory fires involving flammable reagents, follow laboratory fire protocols
Fire Response: Primary fire hazard is flammable reagents and solvents being used or measured (e.g., ethanol, isopropanol, organic extraction buffers). In case of fire, immediately disconnect power to the spectrophotometer if safe to do so. Evacuate the area. Use appropriate extinguisher for the fire type. Do not attempt to extinguish large or rapidly spreading fires; evacuate and call emergency services
Special Protective Equipment for Firefighters: Firefighters should wear self-contained breathing apparatus (SCBA) and full structural firefighting gear (including gloves, boots, helmet, and turnout coat) when fighting fires in laboratory settings, as combustion products may include toxic fumes from burning plastics and electronics

UV-Vis Spectrophotometer (SP-UV1100)

UV-Vis Spectrophotometer (SP-UV1100)

PRIMARY CLINICAL & DIAGNOSTIC USES

SECONDARY & SUPPORTIVE USES

8. Veterinary diagnostic sample analysis: Performs the same range of clinical chemistry, protein quantification, and nucleic acid purity assessments for veterinary patient samples (blood, serum, urine, tissue homogenates) in animal health diagnostic laboratories and veterinary teaching hospitals.

10. Point-of-care test validation in research settings: Serves as a reference method for validating new point-of-care testing devices, rapid diagnostic tests, and laboratory-developed assays during research and development, providing gold-standard spectrophotometric comparison data.

11. Food safety and nutritional analysis: Analyzes food and beverage samples for components such as caffeine, total polyphenols, vitamin C (ascorbic acid), nitrites/nitrates, and food colorants in food safety testing, nutritional labeling, and quality control laboratories.

1. BASIC IDENTIFICATION ATTRIBUTES

Device Type: A single-beam, scanning UV-Vis spectrophotometer with dual-light-source configuration (tungsten and deuterium lamps) for routine laboratory analysis.

Designation: SP-UV1100 UV-Vis Spectrophotometer, DLAB SP-UV1100, Scilogex SP-UV1100.

Key Components:

Tungsten lamp (visible range, 320–1000 nm) and deuterium lamp (UV range, 200–320 nm) with automatic switching

128 × 64 dot backlit LCD display with adjustable brightness and graphical display capabilities

4-position sample compartment for 10 mm path length cuvettes, with optional holders for 5–100 mm path lengths

USB port and parallel port for printer connection

Data storage RAM for up to 200 test data groups and 200 standard curves, with automatic backup

Optional Wave Professional PC software for computer control and advanced analysis functions

2. TECHNICAL & PERFORMANCE PROPERTIES

Optical system: Single beam with dual light source (tungsten and deuterium lamps) with automatic switching based on wavelength

Spectral bandwidth: 4 nm

Wavelength range: 200–1000 nm (standard model)

Wavelength accuracy: ±2 nm

Wavelength repeatability: ≤1 nm

Photometric range: -0.3 to 3.0 A (absorbance) and 0 to 200% T (transmittance)

Photometric accuracy: ±0.003 A at 0.5 A; ±0.006 A at 1.0 A; ±0.5% T at 100% T

Photometric repeatability: ≤0.0015 A at 0.5 A; ≤0.003 A at 1.0 A; ≤0.25% T at 100% T

Stability (baseline drift): ≤0.002 A/h at 500 nm after 2-hour warm-up

Stray light: ≤0.2% T at 220 nm (NaI) and 360 nm (NaNO₂)

Sample compartment: 4-position cuvette holder, standard for 10 mm path length square cuvettes (10 × 10 × 45 mm, 3.5 mL). Optional holders available for 5, 10, 20, 30, 40, 50, and 100 mm path lengths

Data storage capacity: Up to 200 test data groups and 200 standard curves stored in RAM; data retained after power failure (non-volatile)

Wavelength calibration: Automatic wavelength setting with arrow keys for easy calibration and reduced user errors

Output interfaces: USB port for PC connection and data export; parallel port (IEEE 1284) for direct printer connection

Power requirements: 110/220 VAC ±10%, 50/60 Hz, 120 W (user-selectable)

Dimensions (L × W × H): 490 × 360 × 210 mm

Weight: Approximately 12 kg

Quality compliance: Manufactured under ISO 9001 and ISO 13485 quality management systems; CNAS-certified calibration laboratory ensures measurement traceability

3. PHYSICAL & OPERATIONAL PROPERTIES

Construction: Durable, chemical-resistant benchtop enclosure with stable, vibration-dampening base for precise optical measurements

Display: Large 128 × 64 dot backlit LCD screen with adjustable brightness for clear data visualization, including graphic display of spectra and time scans

Operation: Fully standalone operation with all measurement functions executable directly from the instrument; no PC required for routine use

Sample loading: 4-position cuvette holder allows up to four samples to be queued for sequential measurement, improving workflow efficiency

Measurement modes: Photometric mode (fixed wavelength single-point measurement), quantitative analysis mode (standard curve based on up to 9 standards), multi-wavelength scanning mode (up to 10 wavelengths), and time-scan kinetics mode (requires optional software)

Data management: Large-capacity internal data storage for up to 200 test results and 200 standard curves; automatic data backup protects against loss during power interruption; data can be exported via USB

Portability: Benchtop unit weighing 12 kg; not portable but occupies standard laboratory benchtop footprint

Software compatibility: Optional Wave Professional software enables computer control, spectrum scanning (190–1100 nm), time scanning (kinetics up to 12 hours), multi-wavelength scanning (up to 10 wavelengths), and specialized nucleic acid/protein measurement functions

Maintenance: Tungsten and deuterium lamps are user-replaceable; automatic lamp switching optimizes lamp life by using the appropriate lamp for the selected wavelength range

Included accessories: 4 glass square cuvettes (3.5 mL, for visible range >340 nm) and 2 quartz square cuvettes (3.5 mL, for UV range <340 nm), power cord, dust cover, operating manual, and calibration certificate

4. SAFETY & COMPLIANCE ATTRIBUTES

Regulatory Status: General laboratory equipment for in vitro diagnostic (IVD) use; manufactured under ISO 9001:2015 and ISO 13485:2016 quality management systems; CNAS-certified calibration laboratory ensures measurement traceability to national standards

UV Radiation Safety: Deuterium lamp emits UV radiation. Sample compartment cover must remain closed during operation. Interlock mechanism prevents operation with cover open on compliant models

Lamp Safety: Tungsten and deuterium lamps operate at high temperatures (up to 200°C); allow at least 30 minutes cooling before handling or replacement

Electrical Safety: Compliant with IEC 61010-1 for laboratory electrical equipment; grounded power cord; built-in fuse and overload protection

Chemical Safety: Sample compartment and cuvette holder are designed to contain minor spills; exterior surfaces are chemical-resistant to common laboratory reagents (alcohols, dilute acids, dilute bases)

Data Integrity: Automatic backup to non-volatile RAM prevents data loss during power interruption; password-protected calibration settings available (with optional software)

Quality Compliance: Each instrument undergoes individual factory testing; supplied with a calibration certificate traceable to international standards; recommended annual recalibration

5. STORAGE & HANDLING ATTRIBUTES

Storage: Store in a clean, dry, dust-free environment at 15–30°C and ≤80% relative humidity (non-condensing). Cover instrument with dust cover when not in use to protect optical components. Avoid storage near strong magnetic fields, heat sources, or direct sunlight

Installation: Place on a stable, vibration-free benchtop or table away from air vents, windows, and high-traffic areas. Allow at least 10 cm clearance on all sides for ventilation. Do not place near centrifuges or vortex mixers that cause vibration

Warm-up procedure: Turn on instrument and allow 2-hour warm-up before performing critical clinical measurements to achieve specified stability of ≤0.002 A/h. For routine qualitative checks, 30-minute warm-up may be sufficient

Wavelength calibration: Perform automatic wavelength calibration at least weekly or before critical measurements using the auto-setting function accessible via the arrow keys. Use the built-in holmium oxide filter (if equipped) or standard holmium oxide solution for verification

Standard curve preparation: Prepare fresh calibration standards for quantitative analysis daily or as recommended by reagent kit instructions. Store up to 200 standard curves in instrument memory for repeated use; verify curve periodically with a known control standard

Software installation: To install optional Wave Professional PC software, contact manufacturer for compatibility and system requirements. Software enables advanced functions including spectrum scanning, kinetics analysis, and nucleic acid/protein measurement

Periodic verification: Perform photometric accuracy and wavelength accuracy verification monthly using certified reference materials or manufacturer-provided calibration filters. Document results for quality assurance records

6. LABORATORY & CLINICAL APPLICATIONS

1. SAFETY PRECAUTIONS

Hot Surface Hazard: The tungsten lamp and deuterium lamp become extremely hot during operation (surface temperatures can exceed 150°C). Allow lamps to cool for at least 30 minutes before handling or replacement. Do not touch the lamp housing during or immediately after operation

Cuvette Safety: Handle glass and quartz cuvettes with care; breakage can cause cuts from sharp glass shards. Discard broken cuvettes immediately in an appropriate sharps container. Do not use cuvettes with chips or cracks

Sample Containment: Ensure cuvettes are properly seated in the sample holder to prevent tipping or spillage. For volatile or hazardous samples, use sealed cuvettes with caps. Do not overfill cuvettes (maximum volume 3.5 mL for standard cuvettes, fill to approximately 2.5–3.0 mL)

Stability Requirement: Allow the instrument to warm up for 2 hours before performing critical clinical measurements to achieve specified stability of ≤0.002 A/h. Failure to warm up may result in inaccurate results due to baseline drift

Vibration Avoidance: Do not operate the instrument on the same benchtop as centrifuges, vortex mixers, or other vibration-generating equipment. Vibration can cause unstable readings and measurement errors

Training Requirement: Only trained laboratory personnel with appropriate technical background in spectrophotometry and clinical laboratory procedures should operate the SP-UV1100 for clinical diagnostic applications. Users should read and understand the operator's manual before first use

2. FIRST AID MEASURES

UV Exposure to Skin: Prolonged UV exposure may cause skin erythema (redness resembling sunburn). Cool the affected area with cool running water for 15 minutes. If blistering or severe pain develops, seek medical attention

Chemical Splash to Skin: Wash contaminated skin with soap and water thoroughly for at least 5 minutes. Remove contaminated clothing. If irritation, redness, or chemical burns develop, seek medical evaluation. Refer to the specific SDS for the chemical involved

3. FIRE FIGHTING MEASURES

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