Multi-functional Horizontal Electrophoresis Tank (DL-SUB03)

Multi-functional Horizontal Electrophoresis Tank (DL-SUB03)

PRIMARY CLINICAL & DIAGNOSTIC USES

1. High-Throughput PCR Product Confirmation for Infectious Disease Testing

• Primary Use: Provides a large-format multi-functional horizontal electrophoresis tank with interchangeable gel trays (200 × 200 mm, 200 × 130 mm, 130 × 200 mm, 130 × 130 mm, 130 × 65 mm) and multiple comb configurations, enabling high-volume separation of PCR products for infectious disease diagnosis .

• How it helps: For the molecular biologist and clinical laboratory scientist, the DL-SUB03 delivers exceptional sample loading capacity using long gel configurations—allowing confirmation of up to 200 samples per run. For the patient undergoing molecular diagnostic testing for COVID-19, influenza, hepatitis, HIV, or other infectious diseases, high-volume processing reduces turnaround time for test results, enabling earlier diagnosis and treatment initiation.

2. DNA Fragment Analysis and Size Determination

• Primary Use: Supports separation of DNA fragments from 5 to 2500 base pairs with resolution of fragments differing by 10 base pairs, enabling precise molecular weight determination and fragment sizing .

• How it helps: For the molecular geneticist and clinical laboratory scientist, the DL-SUB03’s long gel configuration (200 × 200 mm) provides extended separation distance for high-resolution fragment analysis—enabling accurate sizing of PCR products, restriction fragments, and sequencing samples . For the patient undergoing genetic testing for inherited disorders, precise fragment sizing supports definitive diagnosis and informs clinical management decisions.

3. Restriction Fragment Length Polymorphism (RFLP) Analysis

• Primary Use: Supports large-format restriction fragment analysis for genetic fingerprinting, mutation detection, and population genetics using 1.0 mm and 1.5 mm thickness combs .

• How it helps: For the molecular geneticist, the DL-SUB03’s large gel format provides superior separation of complex restriction fragment patterns—enabling detection of genetic mutations associated with inherited disorders, cancer predisposition, and pharmacogenetic variation . For the patient undergoing genetic screening, high-resolution RFLP analysis supports accurate diagnosis and personalized treatment planning.

4. Multiplex PCR Pathogen Detection and Resolution

• Primary Use: Supports multiplex PCR product resolution using long gels (200 × 200 mm configuration) with sample loads up to 200 samples per run, enabling simultaneous detection of multiple pathogen targets in high-volume testing .

• How it helps: For the clinical microbiologist, the DL-SUB03’s long gel configuration provides extended separation distance for resolving multiple PCR amplicons of different sizes—enabling multiplex detection of respiratory virus panels, gastrointestinal pathogen panels, and sexually transmitted infection panels in high-volume clinical laboratories. For the patient, simultaneous testing for multiple pathogens reduces the number of specimens required and accelerates diagnosis of co-infections.

SECONDARY & SUPPORTIVE USES

1. RNA Denaturing Gel Electrophoresis: Used for formaldehyde-agarose gel electrophoresis for assessing RNA integrity and purity (28S/18S ratio) in molecular diagnostics .

2. Gel Extraction and Purification: Used for separation and visualization of target DNA fragments before gel extraction for downstream applications (e.g., sequencing, cloning).

3. Southern Blotting Sample Preparation: Provides DNA separation for subsequent transfer to membranes for hybridization-based detection.

4. Microbial Pathogen Identification: Used for PCR product confirmation in bacterial, viral, and fungal identification workflows in clinical microbiology .

5. DNA Fragment Size Determination: Supports molecular weight determination of DNA fragments using DNA ladders and size markers with 10 bp resolution .

6. AFLP Analysis (Amplified Fragment Length Polymorphism): Supports large-format fragment separation for genetic fingerprinting and marker-assisted selection.

7. Blood Bank and Transfusion Medicine: Supports DNA-based blood group genotyping and donor screening for blood-borne pathogens.

8. Food Safety and Quality Testing: Supports GMO detection, foodborne pathogen identification, and food authenticity testing.

9. PFGE Sample Confirmation: Used for checking DNA quality and size range before pulsed-field gel electrophoresis for bacterial typing in outbreak investigations.

10. Veterinary Diagnostic Laboratory: Used for PCR product confirmation and nucleic acid analysis for animal health testing .

11. Environmental Microbiology Testing: Used for detection of microbial pathogens in water, soil, and environmental samples by PCR and electrophoresis .

12. Pharmaceutical Quality Control: Used for nucleic acid purity assessment in biopharmaceutical manufacturing .

13. Pharmacogenomics Testing: Supports DNA fragment analysis for genotyping of drug metabolism genes (e.g., CYP450) .

14. Research and Academic Laboratories: Provides large-format electrophoresis for DNA fragment analysis in molecular biology research .

15. Educational and Training Laboratories: Supports teaching of PCR and electrophoresis techniques in medical and graduate training programs .

16. Method Development and Optimization: Used to optimize electrophoresis protocols for clinical implementation .

17. Troubleshooting and Quality Assurance: Supports investigation of PCR failures, contamination events, and assay performance issues .

18. Sequencing Sample Preparation: Used for checking sequencing reaction products before purification .